. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 523 This method for the differential staining of axons we have called the pyridine-silver technique. It is a modification of the Cajal stain which itself stains axons with the greatest clearness in the gray sub- stance of the central nervous system and in the peripheral ganglia. The Cajal method does not, however, clearly differentiate non-meduUated fibers from connective tissue and neuroglia, and fails to bring these axons out with any clearness in the cerebrospinal nerves and in the. Fig. 1. From a cross section of tiie tru
. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 523 This method for the differential staining of axons we have called the pyridine-silver technique. It is a modification of the Cajal stain which itself stains axons with the greatest clearness in the gray sub- stance of the central nervous system and in the peripheral ganglia. The Cajal method does not, however, clearly differentiate non-meduUated fibers from connective tissue and neuroglia, and fails to bring these axons out with any clearness in the cerebrospinal nerves and in the. Fig. 1. From a cross section of tiie trunk of the human vagus nerve some distance below the nodose ganglion. Pyridine-silver. X 1020. fiber tracts of the central nervous system. By the use of pyridine it is impossible to secure a selective staining of the non-raedullated fibers, making these appear black while all other parts of the tissue are light yellow or colorless. The steps of this technique are as follows: The animal is exsanguin- ated and the desired tissue promptly removed and placed in absolute. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Anatomische Gesellschaft. Jena : G. Fischer
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