. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIBERS OF FERTILIZED SEA URCHIN EGGS 489 TABLE I Migration speed of the egg before and after centrifugation (125 g, 3 min) Velocity of egg Species Before centrifugation After centrifugation Temperature A. crassispina H. pulcherrimus ± ± ± ± 26°C 22°C Average and standard deviation based on 20 measurements are given in j/m/s. Potential gradient: V/cm. Estimation of tension and internal viscous resistance of the fibers To estimate tensional force and internal viscous resistance o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIBERS OF FERTILIZED SEA URCHIN EGGS 489 TABLE I Migration speed of the egg before and after centrifugation (125 g, 3 min) Velocity of egg Species Before centrifugation After centrifugation Temperature A. crassispina H. pulcherrimus ± ± ± ± 26°C 22°C Average and standard deviation based on 20 measurements are given in j/m/s. Potential gradient: V/cm. Estimation of tension and internal viscous resistance of the fibers To estimate tensional force and internal viscous resistance of the fibers, the magnitude of external force required for movement of the egg within the perivitelline space was determined by the following procedures. After centrifugation (125 g, 3 min), the egg velocity falling within the perivitelline space under gravitational force was determined in sucrose-sea water solution fol- lowing Hiramoto's method (1954). The time required to pass the distance of 5 ^m (A. crassispind) or 10 yum (H. pulcherrimus} at the center of the space was measured 20-50 min after centrifugation and the velocity was determined. The average often measurements in Anthocidaris eggs and in Hemicentrotus eggs are and respectively. The gravitational force (F) acting on the egg is given as H (1) 8 V) ^ E 3. § 0) • _o d> 2345 Potential gradient (V/cm) FIGURE 9. Relation between the velocity of egg and the potential gradient. Each point represents the average of five measurements. Results obtained from the same egg are shown by the same symbols. The results shown by the same sort of line were obtained from the eggs of the same animal. A. cras- sispina. 26° Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine
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