. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AIR-ABRASIVE MICRODISSECTION 261 prepared by this method from the labial side of the coronal surface only, since the enamel covering the lingual surface was too thin. A second application of this technique has been the microdissection of the prismatic and nacreous layers of the shell of the mollusc, Mercenaria mercenaria. In this instance, however, because of the size and shape of the shell, it was difficult to cut the 1600-micron cross-sections of the shell in the (lillings-Hamco FIGURE 9. Lip portion of shell o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AIR-ABRASIVE MICRODISSECTION 261 prepared by this method from the labial side of the coronal surface only, since the enamel covering the lingual surface was too thin. A second application of this technique has been the microdissection of the prismatic and nacreous layers of the shell of the mollusc, Mercenaria mercenaria. In this instance, however, because of the size and shape of the shell, it was difficult to cut the 1600-micron cross-sections of the shell in the (lillings-Hamco FIGURE 9. Lip portion of shell of Mercenaria mercenaria from which a slab has been partially cut, using a rectangular nozzle (; < ;). This is to be used for the dissection of the prismatic layer. < N = nacreous layer; P = prismatic layer. These were readily prepared, however, by air-abrasive dissection using the ; X ; rectangular nozzle (Fig. 9). In a fashion similar to that described for enamel, the periostracum and the outer one-fourth of the prismatic layer were first removed from cross-sections of the shell, following which a slab of pure pris- matic layer was dissected by air-abrasive cutting along the junction of the prismatic and nacreous layers (Fig. 10). The nacreous layer can be- similarly isolated. The microdissection technique has also been used in the preparation of oriented blocks of mineralized material for electron microscopy and x-ray diffraction studies. For example, 300- to 500-micron-thick coronal or cross-sections of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; R
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
