. Contributions from the Osborn Botanical Laboratory. Plants. Dec, 1921] PAT ON — POLLEN AND POLLEN ENZYMES 491 to 5-cc. portions were added 5 cc. of distilled water and 100 mg. of pollen. For the control the pollen and water were first autoclaved. The tubes were incubated at 37 ° C. for 24 hours or longer (see table 13). The tubes were taken from the incubator and placed simultaneously in ice water, and the failure to solidify, or degree of congealing, during 10 minutes was noted. Many investigators have used gelatin for detecting enzymes of the pepsin type, but the experiments of Malfitano,
. Contributions from the Osborn Botanical Laboratory. Plants. Dec, 1921] PAT ON — POLLEN AND POLLEN ENZYMES 491 to 5-cc. portions were added 5 cc. of distilled water and 100 mg. of pollen. For the control the pollen and water were first autoclaved. The tubes were incubated at 37 ° C. for 24 hours or longer (see table 13). The tubes were taken from the incubator and placed simultaneously in ice water, and the failure to solidify, or degree of congealing, during 10 minutes was noted. Many investigators have used gelatin for detecting enzymes of the pepsin type, but the experiments of Malfitano, Mavrofannis, and Jordan, recently confirmed by Berman and Rettger, seem to indicate that lique- faction of gelatin by an organism is not proof of proteolytic activity. How- ever, since pollen extracts, like pineapple juice, possess this power of lique- faction to a marked degree it has been considered worth while to record the observations. Tests for Trypsin Table 14 Congo red, blood fibrin, 2 cc. N/10 Na2C03, 10 cc. pollen extract (50 mg. in 100 cc. distilled water, unheated and autoclaved), I mg. thymol in each Appearance of Solutions Unheated No change Fibers disintegrated. Liquid very pink No change Fibers less firm. Liquid pinkish Liquid faint pink Fibers disappeared. Liquid red-brown No change Fibers partly disinte- grated, liquid pink Fibers partly disinte- grated, liquid pink Fibers partly disinte- grated, liquid pink Fibers partly disinte- grated, liquid pink Fibers partly disinte- grated, liquid pink Fibers disintegrated, liquid pink No change Autoclaved No change Fibers unaltered Liquid pale, yellow No change No change Fibers unaltered Liquid yellow-brown No change No change No change No change No change No change No change From tables 14-16 it may be seen that corn, goldenrod, Austrian pine, white pine, ragweed, rye, and timothy all gave positive results. Dock gave strongly positive results in the less alkaline medium, while magnolia and goldenrod were str
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