. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HSP GENE EXPRESSION IN OYSTER 165 35 o January 2000 | 30 fjU 1800 D Totten ^ i drift rri D HSP69 EHSC72 * "5 g* 20 S Tolten T 0HSC77 £ 4( §H , V^ Q. 100° T = 1 ^N 800 I 1 9 « > w Sx — 2. •*• 11 10 NX & 600 ^ T •3 — X\X ^^1 H^^^ .Qf. i aly 77 I ' u 0 • ^ ^ M 1 li 2°I m m m 1 % to m .m i i 20C-25C 25C-30C 30C-35C 35C-40C 40C-45C CON 25 29 " " CON 25 29 33 " Temp, range Totten Totten 1 -2m Figure 2. The approximate hours of exposure to elevated (>20°C) air temperatures in oysters at
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HSP GENE EXPRESSION IN OYSTER 165 35 o January 2000 | 30 fjU 1800 D Totten ^ i drift rri D HSP69 EHSC72 * "5 g* 20 S Tolten T 0HSC77 £ 4( §H , V^ Q. 100° T = 1 ^N 800 I 1 9 « > w Sx — 2. •*• 11 10 NX & 600 ^ T •3 — X\X ^^1 H^^^ .Qf. i aly 77 I ' u 0 • ^ ^ M 1 li 2°I m m m 1 % to m .m i i 20C-25C 25C-30C 30C-35C 35C-40C 40C-45C CON 25 29 " " CON 25 29 33 " Temp, range Totten Totten 1 -2m Figure 2. The approximate hours of exposure to elevated (>20°C) air temperatures in oysters at high and low tidal heights for the 2 weeks prior to the summer sampling. The vast majority of the time, oysters experienced temperatures (15-20°C) below the range shown. (to 46°C) during the summer. However, when August oys- ters were heat-shocked at 40°C, they were able to withstand heat shock at 46°C. These results suggest that induction of thermal tolerance is more closely associated with inducible HSP70 family expression. Discussion Our results demonstrate significant phenotypic plasticity in the heat-shock response of Pacific oysters. Massive changes (10- to 100-fold increases) in the amounts of con- stitutively expressed HSP70 proteins were measured in summer as compared to winter. These changes are associ- ated with concomitant increases in thermal limits and set point for HSP69 induction. In oysters at both high and low tidal height, the threshold for complete (, equivalent to levels of HSC77 and HSC72) induction of HSP69 increased 5000. DHSP69 4500 T August 0HSC72 B,HSC77 * 4000 ^^ -•- •s 350° •*" 4n S ,000, T -»- i- ,g i rr^r- | 2S00 - S' 2000 ~t- -t 3 1500 • ^ 1000 - 500 J 0 4- I No data [Vyl 1 1 i. 1 i i \ 1 CON 33 37 40 43 CON 33 37 40 43 TottenO 3m Totten Tidal Height/Temperature Figure 4. Relative levels of HSP70 as determined from western blots for animals sampled in August and later in January. Bars represent the me
Size: 2037px × 1227px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
