. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Vol. 133, No. 3 Vy ^^_^^ \./ December, 1967 THE BIOLOGICAL BULLETIN PUBLISHED BY THE MARINE BIOLOGICAL LABORATORY THE UPTAKE OF NA-22 DURING INDUCTION IN PRESUMPTIVE EPIDERMIS CELLS OF THE RANA PIPIENS GASTRULA1 LESTER G. EARTH AND LUCENA J. EARTH Marine Biolotjical Laboratory, }\\>ods Hole, Massachusetts 02543 Previous investigations (Barth, 1966) have shown that induction of various cell types from presumptive epidermis cells of the Rana pipiens gastrula by sucrose is a two-step process. The sucrose solution prepares th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Vol. 133, No. 3 Vy ^^_^^ \./ December, 1967 THE BIOLOGICAL BULLETIN PUBLISHED BY THE MARINE BIOLOGICAL LABORATORY THE UPTAKE OF NA-22 DURING INDUCTION IN PRESUMPTIVE EPIDERMIS CELLS OF THE RANA PIPIENS GASTRULA1 LESTER G. EARTH AND LUCENA J. EARTH Marine Biolotjical Laboratory, }\\>ods Hole, Massachusetts 02543 Previous investigations (Barth, 1966) have shown that induction of various cell types from presumptive epidermis cells of the Rana pipiens gastrula by sucrose is a two-step process. The sucrose solution prepares the cells for induction by the salt solution in which they are cultured. Induction by the salt solution after sucrose treatment is proportional to the concentration of sodium chloride. The induction produced by lithium chloride in high concentrations for relatively short periods of time is likewise dependent upon the concentration of sodium chloride in the solution used for culturing the cells (Barth and Barth, unpublished data). While our standard solution contains other ions which may possibly complete the process of induction started by sucrose or lithium chloride, the sodium chloride dependency of the process suggested a study of the uptake of Na22. Are the cells so altered by treatment with sucrose or lithium chloride that, upon return to standard solution, sodium ions enter more rapidly than in control cells which have not undergone treatment ? METHODS The procedure for isolating, treating and culturing small aggregates of cells from the gastrula has been described in earlier publications (Barth and Barth, 1967, for references). The concentration of NaCl in the standard solution used for operating is 515 ing. per 100 ml. For treatments of cells the medium was modified as described in detail in tables of data presented below. When aggregates were cultured in order to observe effects of treatments upon differentiation, the NaCl content of standard solution was 450 mg. per 100
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