. The Biological bulletin. Biology; Zoology; Marine biology. Figure 1. Schematic view of the lantern of Eucidaris iribuloides. The lantern is oriented with the tips of the teeth at the bottom and the plumulae at the top. One jaw (j) is stippled and its corresponding tooth (t) is black, co: compass, ipm: interpyramidal muscle, pi: plumula of tooth, r: rotula. Figure 2. Schematic cross section of a jaw and a tooth of Eucidaris tribuloides. br: branch of jaw nerve penetrating jaw, j: jaw, jn: jaw nerve, t: tooth, tl: tooth ligament, arrowhead: suture where two halves of one jaw are connected. For
. The Biological bulletin. Biology; Zoology; Marine biology. Figure 1. Schematic view of the lantern of Eucidaris iribuloides. The lantern is oriented with the tips of the teeth at the bottom and the plumulae at the top. One jaw (j) is stippled and its corresponding tooth (t) is black, co: compass, ipm: interpyramidal muscle, pi: plumula of tooth, r: rotula. Figure 2. Schematic cross section of a jaw and a tooth of Eucidaris tribuloides. br: branch of jaw nerve penetrating jaw, j: jaw, jn: jaw nerve, t: tooth, tl: tooth ligament, arrowhead: suture where two halves of one jaw are connected. For light microscopical histochemistry, fixation was in buffered formalin for several days. Specimens were de- calcified in 1% EDTA solution and embedded in paraffin. Sections were dewaxed and stained with routine methods (Humason, 1979). Staining methods with alcian blue in critical electrolyte concentration are described by Scott and Dorling (1965). For autoradiographic experiments, specimens of Eu- cidaris tribuloides were injected with 3H-glycine to a final activity of 25 fiCi per gram wet weight. The animals were left in seawater for 45 min, 3 h, or 24 h. Jaws with teeth were subsequently dissected, fixed, and embedded by routine methods. Semithin cross sections of the den- tal sHde were covered with Kodak autoradio- graphic stripping film for 4 weeks at 18°C in the dark. The film was developed according to manufacturer's instructions. Counterstaining was with fuchsin and azur II. were kept for months in aquaria wdth artificial seawater and fed algae and aquarium fish food. Appropriate pieces of the lantern were dissected and immediately fixed. Rou- tine fixation was for 18 h in 5% glutaraldehyde in M cacodylate buffer (pH ) containing 5 mMCaCl2; post- fixation was in 1% osmium tetroxide including K3Fe(CN)6 in the same buffer. This method will be called GO-fixation. Some small fibrils, especially in the plumula, were best fixed in a 1% osmium tetroxide sol
Size: 1263px × 1979px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1800, bookdecade1890, booksubjectb, booksubjectzoology
