. Biological structure and function; proceedings. Biochemistry; Cytology. 320 J. A. BERGERON AND R. C. FULLER purified fraction have been studied in some detail [26]. On the basis of the above estimate of particle size, the cytochrome content approaches limiting proportions, that is, i or 2 cytochromes per particle. Several years ago it was reported by Williams [41] that photophos- phorylation might have occurred in experiments with crude extracts of a green sulphur bacterium. The observations in our laboratory indicate that light-dependent uptake of phosphate occurs in the "cleared"


. Biological structure and function; proceedings. Biochemistry; Cytology. 320 J. A. BERGERON AND R. C. FULLER purified fraction have been studied in some detail [26]. On the basis of the above estimate of particle size, the cytochrome content approaches limiting proportions, that is, i or 2 cytochromes per particle. Several years ago it was reported by Williams [41] that photophos- phorylation might have occurred in experiments with crude extracts of a green sulphur bacterium. The observations in our laboratory indicate that light-dependent uptake of phosphate occurs in the "cleared" extract but disappears or is greatly reduced during the course of fractionation. Further >- ^ UJ Q -I < % -y-r-r-t-^^ IN H2S SATURATED ACETONE â IN M TRIS BUFFER, PH Fig. II. Quantitative comparison of the spectral change produced by trans- ferring the photosynthetic pigments from the physiological environment of the pigmented particle into solution in HoS-saturated acetone. w'ork is needed to establish whether or not the phosphate uptake in the crude extracts really represents ATP formation and what relationship the particles have to this activity. Discussion The wide range of opinion which exists concerning the importance of structural organization for photosynthesis is illustrated in a recent sym- posium entitled "The Photochemical ApparatusâIts Structure and Function" {BrookJiaven Symp. Biol, ii, 1958). Structural organization above the molecular level was regarded as essential, or important for efficiency, or irrelevant depending upon the type of data and particular aspect of photosynthesis under consideration. Most of the participants,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : S


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