. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 3N DC Q. ° .08- .06- _ Absorbance / ___ Copper / Zinc / __ Calcium --- Conductivity/ / O) 3- o E ^) 3- | ^ ° 0 ^** ™ e | > > § O | ° FRACTION NUMBER FIGURE 3. Elution profile of the cytosolic fraction of the digestive gland of an intermolt crab showing the partitioning of metals; (A) Sephadex G-75 elution profile column X 65 cm with a flow rate of 30 ml/h of 60 mMTris, pH with 2 mM-2 mercaptoethanol and (B) DEAE—Sephacel ion-exchange col- umn X 10 cm us
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 3N DC Q. ° .08- .06- _ Absorbance / ___ Copper / Zinc / __ Calcium --- Conductivity/ / O) 3- o E ^) 3- | ^ ° 0 ^** ™ e | > > § O | ° FRACTION NUMBER FIGURE 3. Elution profile of the cytosolic fraction of the digestive gland of an intermolt crab showing the partitioning of metals; (A) Sephadex G-75 elution profile column X 65 cm with a flow rate of 30 ml/h of 60 mMTris, pH with 2 mM-2 mercaptoethanol and (B) DEAE—Sephacel ion-exchange col- umn X 10 cm using a gradient generate from 250 ml, 60 mM Tris, pH and 250 ml, 300 mM Tris, pH and both contained 2 mM-2 mercaptethanol. The arrows denote the metallothionein peak that was pooled and subjected to ion exchange chromatography. The peak marked K+ represents the position of the potassium peak (very low molecular weight).. .04- The individual elution profiles of digestive gland cytosol for the six different stages of molting blue crabs show differences in metal distributions that can be correlated with the molting process (Fig. 4). The premolt crabs examined (D!, D2, D3) have a very pronounced metallothionein peak, which is dominated by zinc (Fig. 4A, B, C). Zinc is also present in the high (>60 K, Mr), intermediate (~40 K, Mr), and very low (<1 K, Mr) molecular weight fractions. Copper is present only in the high and metallothionein portions of the profile. In the soft crabs (A2), both zinc and copper are present in the metallothionein region (Fig. 4D), and the relative amount of copper has increased. Otherwise, the distributions of copper and zinc in the elution profiles are the same as before. The metal content of the metallothionein in papershell crabs (B,) is entirely copper with no measureable zinc (Fig. 4E), and the zinc is present only in the high, intermediate, and low molecular weight fractions. Hard shell or intermolt crabs (C4) have a very elevate
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
