. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. VITAL STAINING OF PECTINARIA 441 after staining with gentian violet or methylene blue resulted in the mitochondria concentrating at the extreme centrifugal end of the egg. However, electron microscope studies of stratified sea urchin eggs by Lansing, Hillier and Rosenthal (1952) indicated that two layers of mitochondria stratified, one of high density just above the yolk granule layer and one of low density in the centripetal lipid layer. In similar studies, Gross, Philpott and Nass (1956) reported that the mito- chondria we
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. VITAL STAINING OF PECTINARIA 441 after staining with gentian violet or methylene blue resulted in the mitochondria concentrating at the extreme centrifugal end of the egg. However, electron microscope studies of stratified sea urchin eggs by Lansing, Hillier and Rosenthal (1952) indicated that two layers of mitochondria stratified, one of high density just above the yolk granule layer and one of low density in the centripetal lipid layer. In similar studies, Gross, Philpott and Nass (1956) reported that the mito- chondria were concentrated in a layer centripetal to the yolk, yet they concluded ••-•::;:-v:M WV/.'- ;••:•••••. — 8 FIGURE 25. A composite diagram of the centrifuged oocyte of Pectinaria after GV break- down. The stratified particles were stained by different vital dyes, fluorochromes and cyto- chemical tests. The three zones of the unstained centrifuged egg (A, B and C) are indicated, beginning with zone A at the centripetal pole. 1, Fat droplets. 2, Lipid granular cap. 3, Granular band I. 4, Hyaline zone with diffuse granules. 5, Cortical granules. 6, Granular band II, mitochondria and proteid yolk. 7, Dense proteid yolk. 8, Centrifugal vacuole with basophilic granules. that the mitochondria move to new positions both above and below the clear zone (zone B). Shaver (1957) also reported particles likened to mitochondria lying in an area just beneath the lipid cap (zone A). The relative distribution of particles identified as mitochondria in ascidian eggs seems to follow the same pattern. Reverberi (1956; 1957b) believes there are probably at least two kinds of mitochondria; one type is osmiophilic, does not stain. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Wood
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
