. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 706 B. M. SANDERS AND K. D. JENKINS MT. 10 20 30 Elution Volume (ml) 40 Figure 1. Cytosolic distribution of copper in larvae of the crab Rhilhropanopeiis harrisii exposed to free cupric ion concentrations in sea water. HMW, MT, and VLMW represent high molecular weight, metallothionein, and very low molecular weight pools, respectively. The bars represent fractions which were combined for each pool. apparent molecular weight of 10,000 daltons, and (3) very low molecular weight ligands (VLMW; <5000 daltons) which included m
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 706 B. M. SANDERS AND K. D. JENKINS MT. 10 20 30 Elution Volume (ml) 40 Figure 1. Cytosolic distribution of copper in larvae of the crab Rhilhropanopeiis harrisii exposed to free cupric ion concentrations in sea water. HMW, MT, and VLMW represent high molecular weight, metallothionein, and very low molecular weight pools, respectively. The bars represent fractions which were combined for each pool. apparent molecular weight of 10,000 daltons, and (3) very low molecular weight ligands (VLMW; <5000 daltons) which included molecules too small to be resolved by the column. There was a biphasic relationship between [Cu"^^] in sea water and Cu-thionein and LMW Cu. At [Cu^^] from lO"''^^ to 10 '^^ M copper metabolism appeared independent of external [Cu^^] (Fig. 2a). At these concentrations there was no sig- nificant correlation between external [Cu'^^] and Cu-thionein. Copper associated with the VLMW pool was not detectable at environmental concentrations below 10"° M. At concentrations greater than 10"'°^ M, however, Cu in the metallothionein and the VLMW pools correlated significantly with increases in [Cu^^] in sea water (F = 220; = 1, 2; F = 365; = 1,2). This copper accumulation was reflected in total cytosolic Cu (F = 363; = 1,2). The relationship between Cu associated with the HMW ligands and [Cu^^^] in sea water contrasted with those of the other pools (Fig. 2b). Although Cu associated with these ligands was significantly above background (F = 1096; = 8, 36), it did not correlate with [Cu*^^] in sea water and no accumulation phase was observed (Fig. 2b). We examined the relationships between [Cu^^] in sea water, survival, duration of the zoeal stages, and megalopa weight to determine the physiological affect of changes in intracellular Cu distribution. Free cupric ion concentrations did not correlate with survival or the duration of zoeal development a
Size: 2189px × 1142px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauth, bookcentury1900, booksubjectbiology, booksubjectzoology
