. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Simidiidae 403 was induced to circulate by the action of a jet of compressed air released from a glass jet introduced at the lower end of the wide-bore tube. The wide-bore tube had an internal diameter of 1 to 2 cm., a tube of this size being adopted after a number of trials with tubes of various diam- eters as the one in which the most suitable current could be produced. The capacity of the cylinders was about 5 liters and the compressed air served to aerate
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Simidiidae 403 was induced to circulate by the action of a jet of compressed air released from a glass jet introduced at the lower end of the wide-bore tube. The wide-bore tube had an internal diameter of 1 to 2 cm., a tube of this size being adopted after a number of trials with tubes of various diam- eters as the one in which the most suitable current could be produced. The capacity of the cylinders was about 5 liters and the compressed air served to aerate the water as well as to create the current. The length of the wide-bore tube varied, but this ap- peared to be immaterial provided the open- ing at the bottom was kept free of the debris that eventually accumulated at the foot of the cylinder, and the open end at the top was sufficiently submerged to ensure effective circulation of the water up the tube. The tube for introducing the compressed air into the system had an external diameter of 4 mm., the actual jet being drawn out and slightly slanted so as to project the stream of air bubbles against the wall of the wide-bore tube. The two tubes were held firmly to- gether by means of rubber bands, and pieces of rubber tubing were slipped over those parts which were susceptible to injury and seemed to require protection. The cylinders were first filled with tap water and a culture of algae introduced by the addition of about 250 cc. of water con- taining a dense algal growth. This addition tinged the water in the cylinder green, and all that was subsequently necessary was to replenish the stock of algae, when the green tinge showed signs of disappearing. Some of the water in the cylinder was then removed and replaced by an equal amount of water containing the dense algal culture. It was necessary that the jet should deliver a considerable and steady quantity of air as it was found by experience that any but a momentary interrupti
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Keywords: ., bookcentury1900, bookcollectionbiodiversity, bookleafnumber439
