. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 166 Biological Stains amino groups. Some of them are acid and some basic dyes. There is occasional reference in the biological literature to the use of a benzyl violet, but without any indication as to which of the dyes in question is intended. n50 SPIRIT BLUE C. I. NO. 689 Synonyms: Geniiana blue 6B. Anilin blue, alcohol sohible. Light blue. Lyon blue. Paris blue. This is a mixture of di-phenyl rosanilin chloride: H I N /-\ CI H
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 166 Biological Stains amino groups. Some of them are acid and some basic dyes. There is occasional reference in the biological literature to the use of a benzyl violet, but without any indication as to which of the dyes in question is intended. n50 SPIRIT BLUE C. I. NO. 689 Synonyms: Geniiana blue 6B. Anilin blue, alcohol sohible. Light blue. Lyon blue. Paris blue. This is a mixture of di-phenyl rosanilin chloride: H I N /-\ CI H \/—\ NHj CH3 C32H28N3CI; Mol. Wt. and tri-phenyl pararosanilin chloride:. CI I I \_/ H \/-\ N_/-\ "I \_/ H C37H30N3CI; Mol Wi. {Basic dyes absorption maximum of spirit blue 2R about 581 in alcohol.) Solubility at 26°C: in water nil; in alcohol Lynch (1930) mentioned Lyons blue (undoubtedly this dye) as a satisfactory counterstain to carmine in bulk staining of protozoa and other small animals and embryos. It has, moreover, been re-, ported by others as a good contrast stain for carmine, bringing out nerve fibers well in embryonic tissue; for this purpose it is used in strong alcoholic solution. Quite recently, Knaysi (1942) has mentioned this dye as one whose color base can be used in de- termining hydrolysis of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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