. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ELECTRICAL PROPERTIES OF STARFISH EGGS 155 fective after the indented plasma membrane had formed a tight sleeve around the electrode (as in Figure le) and was allowed to remain in this condition for a short while. Figure 2 illustrates eggs with one or two electrodes that have penetrated into the FIGURE 1. Photomicrographs showing apparent entry of a micropipette into an egg of Astcrias forbcsii held by a "sucking" pipette. Magnification, 104 X. The micropipette, filled with isotonic XaCl-KCl solution co
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ELECTRICAL PROPERTIES OF STARFISH EGGS 155 fective after the indented plasma membrane had formed a tight sleeve around the electrode (as in Figure le) and was allowed to remain in this condition for a short while. Figure 2 illustrates eggs with one or two electrodes that have penetrated into the FIGURE 1. Photomicrographs showing apparent entry of a micropipette into an egg of Astcrias forbcsii held by a "sucking" pipette. Magnification, 104 X. The micropipette, filled with isotonic XaCl-KCl solution containing chlorphenol red, is pushed through the vitel- line membrane and indents the underlying surface (plasma membrane) forming a large conical depression (d). After about two minutes the depression closes over the pipette (c) and the latter appears to be within the cytoplasm. However, injection of fluid (/ to h) shows that the walls of the depression had formed a tight sleeve around the shaft of the pipette. The fluid ex- pands this sleeve and stretches the vitelline membrane, flowing out through the latter. Upon removal of the pipette (i to k) the remaining fluid is expelled as the egg rounds-up within some two minutes. The same egg with a fertilization membrane elevated at two minutes after fertili- zation is shown in /.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology