. The Canadian field-naturalist. 2004 Kemp and Lacroix: Seed Bank and vSeed Viability 107. Figure 2. Map of all Prince Edward Island sites used in this study. Study sites are depicted as squares on both maps. All five sites shown in (A) are located in the national park: 1. Covehead Pond, 2. Western Wetland, 3. Dune Slack. 4. East Marsh B, and 5. East Marsh A. (B) Location of sites on Prince Edward Island with black square highlighting the specific area shown in (A). viable will stain pink/red. Complete achenes (contain- ing seeds) were placed on a piece of filter paper in a petrie dish, and le
. The Canadian field-naturalist. 2004 Kemp and Lacroix: Seed Bank and vSeed Viability 107. Figure 2. Map of all Prince Edward Island sites used in this study. Study sites are depicted as squares on both maps. All five sites shown in (A) are located in the national park: 1. Covehead Pond, 2. Western Wetland, 3. Dune Slack. 4. East Marsh B, and 5. East Marsh A. (B) Location of sites on Prince Edward Island with black square highlighting the specific area shown in (A). viable will stain pink/red. Complete achenes (contain- ing seeds) were placed on a piece of filter paper in a petrie dish, and left overnight to soak in distilled water. The next day, when the seeds were fully imbibed with water, they were dissected out of the achene fruit wall and seed coat. After removal of the seed coat, the embryos were placed in a 1% TTC solution for two hours at 35°C, as prescribed for dicotyledonous seeds. The embryos were then evaluated for viability according to the scheme developed in Grabe (1970). As a general rule, the radicle, shoot tip and cotyledons must stain pink/red in order for the seed to be con- sidered viable. Statistical Analysis The viability of seeds in the soil was compared graphically for vertical distribution and sampling dates. Statistical analysis was not possible on these data be- cause there was no replication. Due to the vulnerable status of the plant, sampling was limited by restrictions on collecting imposed by Parks Canada. The vertical distribution of seeds and fruit walls within the soil was compared using Kruskal-Wallis tests (non parametric equivalent of ANOVA). All data from the five sam- pling sites on PEI were pooled for Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ottawa Field-Naturalists' Club. Ottawa, Ottawa Field-Naturalists' Club
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