. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. O2) KCN, IAA ON REGENERATION 185 TABLE VI Simultaneous effects of high oxygen tension and cyanide on survival (infractions') and regeneration (in average number of new segments per worm} 2 days 4 days 6 days 9 days 14 days Under oxygen Tap water 30/30 sm. blastema 23/30 0 seg. 4/30 0 seg. no survivor lO-^ M KCN 30/30 sm. blastema 30/30 2 seg. 28/30 3 seg. 21/30 4 seg. no survivor 10-« M KCN 30/30 sm. blastema 29/30 1 seg. no survivor 10-3 M KCN no survivor Under air Tap water 30/30 blastema 28/30 4 seg. 28/30 13 seg. 27/30 2
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. O2) KCN, IAA ON REGENERATION 185 TABLE VI Simultaneous effects of high oxygen tension and cyanide on survival (infractions') and regeneration (in average number of new segments per worm} 2 days 4 days 6 days 9 days 14 days Under oxygen Tap water 30/30 sm. blastema 23/30 0 seg. 4/30 0 seg. no survivor lO-^ M KCN 30/30 sm. blastema 30/30 2 seg. 28/30 3 seg. 21/30 4 seg. no survivor 10-« M KCN 30/30 sm. blastema 29/30 1 seg. no survivor 10-3 M KCN no survivor Under air Tap water 30/30 blastema 28/30 4 seg. 28/30 13 seg. 27/30 23 seg. 27/30 31 seg. lO"6 M KCN 18/18 blastema 18/18 6 seg. 18/18 18 seg. 18/18 25 seg. 18/18 33 seg. 10-" M KCN 15/15 blastema 12/15 3 seg. 10/15 9 seg. 10/15 11 seg. 10/15 12 seg. ID"3 M KCN 29/30 no blastema no survivor survival and regeneration, worms were allowed to regenerate in various concen- trations of sodium iodoacetate: 1O3 M, 1O4 M. 1Q-5 M, 1O6 M, 1O7 M, and 10'8 M NalAc. Five intact worms in 5 X 10~3 M NalAc showed decreased activity after six hours, and were dead at thirty-six hours. In the other concentrations intact worms survived as well as but no better than the regenerating worms. The group of thirty worms in 1O3 M solution showed high mortality after three days, but the few survivors maintained normal morphogenesis. The worms in 10~4 M solution showed high mortality after six days, but six of the thirty worms survived for twenty-four days and maintained normal regeneration. Mortality in the other groups was low. Rates of progress through various stages of regeneration were very nearly the same for all concentrations of iodoacetate (Fig. 2), but whereas rate of later differentiation in tap water reached a peak between thirteen and seventeen days after cutting, the rate of later differentiation in each of the iodoacetate solutions TABLE VII Effect of dilute iodoacetate upon rate of oxygen consumption Sample Large worms (over cm.) Small wor
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
