Archive image from page 182 of The cytoplasm of the plant. The cytoplasm of the plant cell cytoplasmofplant00guil Year: 1941 Chapter XIV 167 The Vacuolar System longing to the tetragonal system; sometimes as crystal dust. The crystals of calcium sulphate are also very frequently found (Clos- terium, Spirogyra). Fig. 112. — Lilium candidum. Lipide concre- tions within the vacuoles. A, in epidermal cells of the bulb; small granules (1) agglomerate into mulberry-shaped masses (2), these, B. in older scales become large bodies which are globular with a denser center (4) or of radiating crysta
Archive image from page 182 of The cytoplasm of the plant. The cytoplasm of the plant cell cytoplasmofplant00guil Year: 1941 Chapter XIV 167 The Vacuolar System longing to the tetragonal system; sometimes as crystal dust. The crystals of calcium sulphate are also very frequently found (Clos- terium, Spirogyra). Fig. 112. — Lilium candidum. Lipide concre- tions within the vacuoles. A, in epidermal cells of the bulb; small granules (1) agglomerate into mulberry-shaped masses (2), these, B. in older scales become large bodies which are globular with a denser center (4) or of radiating crystalline structure (3). C, in drying outer scales, these break up and myelin figures (5) appear which finally, in dead cells, form masses of wound up threads (6). Vacuolar pH and rH:- It is very difficult to obtain an exact idea of the vacuolar pH. It has been seen indeed that the change of color of the vital dyes has only a very questionable value. Then, too, the indicators of pH do not penetrate into the vacuoles and are besides very toxic. However, evaluations have been attempted by Crozier, Haas and Irwin, who sought to extract the vacuolar sap of certain algae, such as Valonia, in which the articulations are oc- cupied by an enormous vacuole. These evaluations have shown that the vacuolar H in Valonia has an acid reaction (). Crozier, Haas and Schmidt have used an interesting method by making the anthocyanin pigments serve as indicators. These pig- ments can be extracted from the petals and in mixing them with buffer solutions of known 2?H, all their different possible colors may be obtained and consequently the coloring which the petals show naturally may be related to a known pH. The results obtained by this method inform us as to the vacuolar pH. It scales from We have been able with Gautheret to find a way to evaluate the vacuolar pH but only qualitatively. Clark and Perkins have
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