. The Biological bulletin. Biology; Zoology; Marine biology. 32 D. J. CRISP AND B. S. PATEL In some of the experiments the barnacles were fed liberally on Artemia larvae, and in others no food was given, a change of water being made only once a day. Both the fed and the starved animals were maintained at a series of temperatures. One series was kept in a thermostatically controlled refrigerator at 3 ± 1° C, a second in a thermostatically controlled cool cabinet at 5 to 7° C, a third set in a cool basement, the temperature of which differed by only 1-2° C. from that of the mean sea water temper


. The Biological bulletin. Biology; Zoology; Marine biology. 32 D. J. CRISP AND B. S. PATEL In some of the experiments the barnacles were fed liberally on Artemia larvae, and in others no food was given, a change of water being made only once a day. Both the fed and the starved animals were maintained at a series of temperatures. One series was kept in a thermostatically controlled refrigerator at 3 ± 1° C, a second in a thermostatically controlled cool cabinet at 5 to 7° C, a third set in a cool basement, the temperature of which differed by only 1-2° C. from that of the mean sea water temperature, and a fourth in a laboratory where the temperature remained considerably higher, ranging between 15-21° C. In order to study the influence of the parasite Hemioniscus balani on the moult- ing rhythm, infected specimens of B. balanoides L. and of Elminius modestus Darwin were collected from Brixham, S. Devon, where a high level of infection occurs (Southward and Crisp, 1954). Infected specimens of B. amphitrite var. denticulata Broch and B. perforatus Bruguiere were collected from the warm water docks at Swansea and from the coast of the Gower Peninsula, respectively. For these experiments animals were isolated in dishes and fed on Artemia larvae; subsequent examination revealed whether they were infected. Seasonal Variation in the Moulting Rhythm Darwin (1854) in his monograph on Cirripedia mentioned that he was informed by a Mr. Peach that off the coast of Cornwall barnacle exuviae were most abundant in April-May and again in September. The specimens sent to Darwin included. I SEPT | OCT | NOV | DEC | JAN "p? Figure 1. Seasonal variation in the moulting rhythm of groups of B. balanoides L. collected at approximately fortnightly intervals during the years 1954-57. Periods when fertilisation is taking place are shown by dark rectangles, and periods when liberation takes place are shown by clear rectangles. B. balanoides L., B. perforatus Bruguiere and Chthamalus


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