. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . break up into shortalmost coccus-like elements (). Staining.—The bacillus malleistains with difficulty with the ani-line colors, best when the aqueoussolutions of these dyes are madefeebly alkaline; it is decolorizedby Grams method. This bacilluspresents the peculiarity of losingvery quickly in decolorizing solu-tions the color imparted to it bythe aniline staining solution. Forthis reason it is diflScult to stain in sections. Loffler recommends his alkaline methylene-blue solutionfor staini
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . break up into shortalmost coccus-like elements (). Staining.—The bacillus malleistains with difficulty with the ani-line colors, best when the aqueoussolutions of these dyes are madefeebly alkaline; it is decolorizedby Grams method. This bacilluspresents the peculiarity of losingvery quickly in decolorizing solu-tions the color imparted to it bythe aniline staining solution. Forthis reason it is diflScult to stain in sections. Loffler recommends his alkaline methylene-blue solutionfor staining sections, and for decolorizing, a mixture containing 10 distilled water, 2 drops of strong sulphuric acid, and 1 drop of a 5per cent, solution of oxalic acid; thin sections to be left in this acidsolution for five seconds. Biology.—A non-motile bacillus, whose molecular movements areso active that they have often been taken for motility. It is aerobic,but moderate multiplication occurs in the depths of culture well on culture media at 37° C. Development takes place. Fig. 142.—Glanders bacilli. Agar 1100 diameters. 400 GLANDERS BACILLUS slowly at 22° C. and ceases at 43° C. The bacillus does not formspores. Exposure for ten minutes to a temperature of 55° C, or forfive minutes to a 3 to 5 per cent, solution of carbolic acid, or for twominutes to a 1 to 5000 solution of mercuric chloride destroys its a rule the bacilli do not grow after having been preserved in a desic-cated condition for a week or two; in distilled water-they may livetwenty-five days. It is doubtful whether the glanders bacillus findsconditions in nature favorable to a saprophytic existence. A solution of chlorinated lime, containing 1 part of free chlorine per1000, is useful as a disinfectant of stables and utensils; it kills the bacillusin from one to two minutes. Strong sodium carbonate solution (washingsoda) is also useful. Cultivation.—(For obtaining
Size: 1576px × 1586px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauth, bookcentury1900, bookdecade1910, bookidcu31924000235212
