. Cytochemistry: a critical approach. Cells. USE OF CHROMOGENIC REAGENTS 99 degree in the visible. An exception to this is the very interesting study which Thorell (1947) has made of the formation of hemo- globin during the development of red blood cells. A typical example of the work of Caspersson is illustrated by Fig. 5. 2200 2400 2600 2800 3000 3200 Fig. 5. Extinction coefficients for a point on a chromosome (upper curve) and in the cytoplasm (lower curve) of a Tradescantia pollen mother cell. The absorption at 2800 is mainly caused by protein, and at 2600 , mainly by nu
. Cytochemistry: a critical approach. Cells. USE OF CHROMOGENIC REAGENTS 99 degree in the visible. An exception to this is the very interesting study which Thorell (1947) has made of the formation of hemo- globin during the development of red blood cells. A typical example of the work of Caspersson is illustrated by Fig. 5. 2200 2400 2600 2800 3000 3200 Fig. 5. Extinction coefficients for a point on a chromosome (upper curve) and in the cytoplasm (lower curve) of a Tradescantia pollen mother cell. The absorption at 2800 is mainly caused by protein, and at 2600 , mainly by nucleic acid. After Caspersson, 1950, page 66. General Principles Involved in the Use of Chromogenic Reagents There are a wide variety of agents which may be used in order to produce an absorption of light by proteins or nucleic acids in various parts of the spectrum. Up to the present these agents have been almost entirely restricted to the development of ab- sorption bands in the visible region of the spectrum. It seems likely that in the future attempts will be made to use reagents. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Danielli, J. F. (James Frederic), 1911-. New York Wiley
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