. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 JORAM PIATIGORSKY AND ALBERT TYLER method of Martin and Ames (1961) and these values are in close agreement with those of Slater and Spiegelman (1966a). RESULTS 1 ) Retention oj radioactivity b\ Lytechinus pictus after receiving an injection j H3-uridine To test the retention of radioactively labeled uridine injected into the hody cavity of the adult female, the following experiment was performed. Two sea. 2 3 4 5 6 7 14 DAYS AFTER H3-5-URIDINE INJECTION 21 28 FIGURE 1. Retention of radioactivity by Lytechinus pictus aft
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 JORAM PIATIGORSKY AND ALBERT TYLER method of Martin and Ames (1961) and these values are in close agreement with those of Slater and Spiegelman (1966a). RESULTS 1 ) Retention oj radioactivity b\ Lytechinus pictus after receiving an injection j H3-uridine To test the retention of radioactively labeled uridine injected into the hody cavity of the adult female, the following experiment was performed. Two sea. 2 3 4 5 6 7 14 DAYS AFTER H3-5-URIDINE INJECTION 21 28 FIGURE 1. Retention of radioactivity by Lytechinus pictus after receiving an injection of H3-5-uridine. Two females were induced to spawn by injection of potassium chloride and then injected with 125 /xc. of H3-5-uridine (sp. act. 25,000 c/M) in ml. artificial sea water. They were maintained in the same plastic box (16 cm. X 35 cm.) containing 1500 ml. artificial sea water. Duplicate aliquots of the sea water were removed at the specified times and assayed for radioactivity. urchins, weighing approximately 15 grams apiece, were each injected with 125 ^,c. of H3-5-uridine in ml. artificial sea water. They were placed into the same plastic box containing 1500 ml. of artificial sea water but lacking eel grass. The sea urchins were kept rocking gently, as usual, and duplicate samples of the medium were removed and assayed for radioactivity at the indicated times. The sea water was not changed for the three-week duration of the experiment. The results are illustrated in Figure 1. For the first 12 hours less than \% of the injected radioactivity appeared in the medium. By 24 hours, however, almost 5% could be accounted for in the sea water. This value did not increase during the next 21 days. A slight decrease was observed in the third week. It is evident then that sea urchins retain most of the nucleoside introduced into their body cavity under these conditions. It is of. Please note that these images are extracted fro
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
