. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 4. Standardization of evisceration factor bioassay on two isolated pharyngeal retractor muscles (PRM) of Thy one. Quantal responses are related to half-log-unit dilutions of haemal system extracts; quantal response of undiluted extract defined as 10. Doses ( ml) administered to both PRMs: top records—undiluted haemal sj^stem extract (HS) ; middle records—3-fold dilution of HS (HS/3) ; bottom records—10-fold dilution of HS (HS/10). Note two types of responses: (A) delay between dose and contraction increases as dose
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 4. Standardization of evisceration factor bioassay on two isolated pharyngeal retractor muscles (PRM) of Thy one. Quantal responses are related to half-log-unit dilutions of haemal system extracts; quantal response of undiluted extract defined as 10. Doses ( ml) administered to both PRMs: top records—undiluted haemal sj^stem extract (HS) ; middle records—3-fold dilution of HS (HS/3) ; bottom records—10-fold dilution of HS (HS/10). Note two types of responses: (A) delay between dose and contraction increases as dose de- creases; (B) rate of tension development increases with dose. Extract added to the bath at upward-pointing arrows: washout at downward-pointing arrows; rectilinear recordings. TABLE II Relative activity of evisceration factor (EF), and concentration of acetylcholine (ACh), in tissue extracts from uneviscerated Thyone Tissue EF (relative activity*) ACh (moles/liter) Haemal system Respiratory tree Mesentery- Gut Gonad PRM 10 1 1 io-i2-io-10 10-9-10-" lO-9 -ID"8 10~9-10-8 io-i2-io-10 10-8-10~7 * Undiluted haemal system extract (10 mg/ml) is the standard preparation with a defined relative activity: 10. The relative activities of other undiluted extracts are given by the amount of haemal system extract necessary to match their effects. Thus, the activity of 10 mg respiratory tree equals that of 1 mg haemal system extract (see Fig. 4).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
