. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 98 BACTERIOLOGY with alcohol and bichloride and dried. An elastic bandage is applied about the arm to distend, the veins. A sterile needle attached to a special sterilized blood pipette is thrust into the vein and the desired amount of blood collected (see Fig. 37). It may be allowed to clot if sterile serum is desired, or it may be defibrinated by stirring with the glass rod if a mixture of corpuscles and serum is desired, o


. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 98 BACTERIOLOGY with alcohol and bichloride and dried. An elastic bandage is applied about the arm to distend, the veins. A sterile needle attached to a special sterilized blood pipette is thrust into the vein and the desired amount of blood collected (see Fig. 37). It may be allowed to clot if sterile serum is desired, or it may be defibrinated by stirring with the glass rod if a mixture of corpuscles and serum is desired, or it may be kept in the fluid state by the ad- dition of sterile 10 per cent solution of sodium citrate so that the final mixture may contain i per cent of citrate. The bandage is removed from the arm before the needle is withdrawn. Pressure over the wound with cotton wet in alcohol for five minutes prevents sub- cutaneous hemorrhage. No dress- ing is required. The inlet to the blood pipette is closed by kinking the rubber tube. The blood or the serum is subsequently handled by means of sterilized pipettes, and most conveniently by means of Pig. 37.—Pipette with needle at- the Pastcur bulb pipettcs. (See tached for drawing human blood Tjoge 22 ^ from a vein for use in culture media. H 8 oov The glass rod inside is used to defi- Blood from Small laboratory rina e e 00 . animals serves as well as human blood for most purposes. It may be drawn from the carotid artery by aseptic technic into a special blood pipette, the lower end of which is drawn out into a capillary ,which is inserted directly into the artery (see Fig. 38). This blood may be defibrinated, ci- trated or allowed to Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original MacNeal, Ward J. , 1881-1946; Williams, Herbert Upham, 1866-. Philadelphia,


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Keywords: ., bookcentury1900, bookdecade1920, booksubjectbacteri, bookyear1920