A practical treatise on medical diagnosis : for students and physicians . y to pneumonia. The bacillus coli communis is found inabscesses about the peritoneum and in purulent peritonitis, the amoebadysenterica in abscess of the liver and secondary abscess of the pleuraand lung. It was found in an abscess of the jaw by Flexner. Themicrococci are detected by the staining methods. The method of Gramis the most satisfactory. After a cover-glass has been prepared and placed in Ehrlich-Weigertssolution of gentian-violet and aniline water, it is put into a solution ofiodine and iodide of potassium fo
A practical treatise on medical diagnosis : for students and physicians . y to pneumonia. The bacillus coli communis is found inabscesses about the peritoneum and in purulent peritonitis, the amoebadysenterica in abscess of the liver and secondary abscess of the pleuraand lung. It was found in an abscess of the jaw by Flexner. Themicrococci are detected by the staining methods. The method of Gramis the most satisfactory. After a cover-glass has been prepared and placed in Ehrlich-Weigertssolution of gentian-violet and aniline water, it is put into a solution ofiodine and iodide of potassium for two or three minutes. A dull red-brown color is produced. It is then rinsed in absolute alcohol for sometime. The micro-organisms are stained dark blue. The iodide ofpotassium solution is: Iodine, 1 part; iodide of potassium, 2 parts;distilled water, 300 parts. By this method the various forms of micro-organisms just indicated are readily brought out. The Pyogenic Bacteria. 1. Staphylococcus PyogenesAureus.—This micro-organism is found in acute abscesses and boils,. Pas with staphylococcus. X 800. (Flugge.) sometimes also in infectious osteomyelitis and ulcerative enters the tissues through abrasions or the hair follicles. Morphology. In cover-glass preparations they appear as small roundbodies scattered among the pus-cells, rarely within them, single, in pairsor clusters. They stain readily with the basic aniline dyes. (See PlateI., Fig. 2, b; and Fig. 15.) Biological properties. It is aerobic, facultative anaerobic, grows inmilk, meat infusions, gelatin, or agar at 18° C. Death-point is 56° to58° C. after ten minutes exposure. Growth. Make plate cultureson agar-agar. After twenty-four hours in the incubator the plate willbe studded with yellow or orange-colored colonies, round, moist, andglistening. In a gelatin stab culture, liquefaction occurs in thirty-six
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