. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 15 18 21 24 40 2 o Q. O o 30 20 10 B. 9 12 15 18 Time (h) 21 24 Figure 4. Incorporation of [3H]uridine in the presence or absence of emetine or anisomycin as a function of time after addition of GABA (ICT6 M). The larvae were pulsed for 20 min with radiolabeled nucleoside ( ^Ci/ml) at the times indicated. Mean values slightly displaced on the abscissa for clarity. (A) I0-day-old larvae were used. Where indicated, emetine (9 p.\l) was added 30 min prior to the addition of GABA. No emetine or GABA (diamonds); emetine
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 15 18 21 24 40 2 o Q. O o 30 20 10 B. 9 12 15 18 Time (h) 21 24 Figure 4. Incorporation of [3H]uridine in the presence or absence of emetine or anisomycin as a function of time after addition of GABA (ICT6 M). The larvae were pulsed for 20 min with radiolabeled nucleoside ( ^Ci/ml) at the times indicated. Mean values slightly displaced on the abscissa for clarity. (A) I0-day-old larvae were used. Where indicated, emetine (9 p.\l) was added 30 min prior to the addition of GABA. No emetine or GABA (diamonds); emetine with no GABA (rectangles): no emetine, plus GABA (triangles); emetine plus GABA (circles). (B) 9-day- old larvae were used. Where indicated, anisomycin (200 nl\f) was added 60 min prior to the addition of GABA. No anisomycin or GABA (dia- monds); anisomycin with no GABA (rectangles): no anisomycin, plus GABA (triangles): anisomycin plus GABA, (circles). concentration of GABA; virtually all of the larvae were attached within 20 min, with no inhibition by emetine (Fig. 5B). Although emetine did not inhibit the initial rate of attachment of the larvae induced by GABA. the larvae failed to maintain their plantigrade attachment (Fig. 5A. B), and progressively more were found on their sides, ap- parently due to the toxic effect of prolonged exposure to emetine. There also was some attachment in the presence of emetine when GABA was absent (Fig. 5A. B). Prolonged exposure of larvae to emetine proved lethal. Even before any mortality was observed, larvae treated with 9 fiM emetine appeared to spend more time on the bottom of the test vial than larvae in control vials. By 36 h after the addition of emetine (9 nAf) both in the presence and absence of GABA (ca. 20 larvae/ml), few larvae were swimming and many appeared dead, while in the control vials lacking GABA, many of the larvae remained swimming and virtually all remained alive. All the larvae were dead by 54 h in the presence of 9
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
