. Bulletin. Science; Natural history; Natural history. â ft" â !⢠*" tXi. Fif;uir I. l3o\cr solo uilh skin tumors (arrows) on pectoral and caudal Tins and abdomen. Fi^'iirv 2. have been reported as etiologic agents and sewer effluent or pollution has been suggested as the source (Wellings et ai. 1965; Carlisle, 1969: Young, 1964; Russell and Kotin, 1957; Turner and Strachan, 1969). An unidentified cell type (altered fish cell or unicellular parasite having nucleus and mitochondria) labeled "X-cell" has been observed in tumors of various species of flounder (Brooks et al.,
. Bulletin. Science; Natural history; Natural history. â ft" â !⢠*" tXi. Fif;uir I. l3o\cr solo uilh skin tumors (arrows) on pectoral and caudal Tins and abdomen. Fi^'iirv 2. have been reported as etiologic agents and sewer effluent or pollution has been suggested as the source (Wellings et ai. 1965; Carlisle, 1969: Young, 1964; Russell and Kotin, 1957; Turner and Strachan, 1969). An unidentified cell type (altered fish cell or unicellular parasite having nucleus and mitochondria) labeled "X-cell" has been observed in tumors of various species of flounder (Brooks et al., 1969) and also in tumors of Dover sole (Mearns and Sherwood, 1974a). On several occasions Microstomiis pacificits were collected near sewage outfalls in Santa Mon- ica Bay, Newport Beach, and Port Hueneme, California. All of the five specimens examined were small, 82 to 122 mm standard length and weighed to g. Skin tumors occur on the eyes, mouth, fins, and body. They are roughly circular, to 10 mm in diameter. The mass of the tumor is raised above the level of the surround- ing normal tissue and extends slightly into the epi- dermis (Fig. 2). The surface of the tumor is furrowed; on some tumors the furrows radiate outward. Mycoplasma-like organisms were found in all tumors (nine) of the five specimens. For electron microscopy, tissues were fixed in sodium cacodylate buffered glutaraldehyde and postfixed in osmium tetroxide also buffered with sodium cacodylate. Tissues were dehydrated in graded series of ethanol and embedded in Epon 812. Sections were stained with aqueous uranyl acetate and lead citrate. Electron microscopical observations of the epi- dermal papillomas from M. pacifictts reveal or- ganisms (Figs. 3â1) having the morphological characteristics of mycoplasmasâthat is, absence of a cell wall, plasticity of body form, a triple layered membrane surrounding the organism, no apparent nuclear membrane, no membranous or- ganelle, and size range of
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