. Carnegie Institution of Washington publication. Anatomy and Development of Posterior Lymph Hearts of Turtles. 81 Mallory's connective-tissue stain is also very good. Many of the older embryos had the blood-vessels injected with India ink. Embryos up to 26 days were imbedded in paraffin and cut IO/JL to 20« thick. Older embryos were cut by the celloidin method, about 25^ in thickness and mounted on lantern slides. One series of embryos was cut into 50," sections, and another series of older stages was cut into sections iooj« in thickness. Wax and blotting paper, as well as graphic recons


. Carnegie Institution of Washington publication. Anatomy and Development of Posterior Lymph Hearts of Turtles. 81 Mallory's connective-tissue stain is also very good. Many of the older embryos had the blood-vessels injected with India ink. Embryos up to 26 days were imbedded in paraffin and cut IO/JL to 20« thick. Older embryos were cut by the celloidin method, about 25^ in thickness and mounted on lantern slides. One series of embryos was cut into 50," sections, and another series of older stages was cut into sections iooj« in thickness. Wax and blotting paper, as well as graphic reconstructions, were made whenever necessary. ab a~z. ANATOMY OF THE POSTERIOR LYMPH HEARTS OF TURTLES. Lymph hearts were discovered in turtles by Joh. Mtiller in 1839. He describes them in the green turtle (Chelonia mydas] as a pair of rounded organs, more or less flattened dorsoventrally, lying just caudad of the upper end of the ilium, one on each side of the body. They rest upon the origin of the semiten- dinosus muscle of each side and are bordered laterally by the biceps and caudally by the semimembranosus muscles. Lymph channels from the pos- terior extremities and caudal portion of the body open into their posterior ends. Their pulsations are irregular, occur- ring at the rate of 3 or 4 times per minute, and are not neces- sarily synchronous for the two hearts. Their inner wall is smooth and their cavity is free and not broken by trabeculse or septa, as it is in the land turtle. The openings of the afferent and of the efferent ducts are guarded by valves. They open, by means of a short duct, into the vein that runs forward along their mesial border to become the pos- terior renal advehent vein of each side. In the large land turtle (Testudo elephantina), described by Fritsch (1874), the lymph hearts are ovoid in form with a longitudinal diameter of 38 mm. and a width of 20 mm. in the anterior half, and 15 mm. in the posterior half. Their walls contain striated muscle fiber


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