. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 18 W. R BATtS. Figures I and 2. Transmission electron micrographs of a sectioned Mnlt;iilti ri'inriilnnni.\ follicle cell (1; and a sectioned M rclornfrmiiix gastrula (2). The swirl patterns of follicle cell droplets (d) are evident in (1! and a test cell (tc) is seen within the perivitelline space (ps) in (2). X 3300 in (1) and in (2). Immunocytochemistry M. retortiformis and B. villosu eggs were prepared for immunocytochemistry, as previously described by Mita-Miyazawa et al. (1987). Eggs were immersed for 20 min in absolu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 18 W. R BATtS. Figures I and 2. Transmission electron micrographs of a sectioned Mnlt;iilti ri'inriilnnni.\ follicle cell (1; and a sectioned M rclornfrmiiix gastrula (2). The swirl patterns of follicle cell droplets (d) are evident in (1! and a test cell (tc) is seen within the perivitelline space (ps) in (2). X 3300 in (1) and in (2). Immunocytochemistry M. retortiformis and B. villosu eggs were prepared for immunocytochemistry, as previously described by Mita-Miyazawa et al. (1987). Eggs were immersed for 20 min in absolute methanol, and then for 20 min in cold absolute ethanol. Fixed eggs were infiltrated with 50% polyester wax (BDH Limited, Poole, England): ab- solute ethanol for 1 h at 40°C and then infiltrated with 100% polyester wax for 1 h at 40°C. Specimens were embedded in BEEM capsules, and 8-/jm sections were cut from the blocks. Sections were mounted on gelatin- coated coverslips, de-waxed through a graded series of ethanol dilutions (100%; 90%; 80%; 70%; 50%; 30%), and rinsed in phosphate buffered saline (PBS). The specimens were incubated with a monoclonal antibody (1:25 dilution of NN18 from Sigma Chemicals) for 1 h at room temperature, washed with PBS, and incubated for 50 min in a 1:60 dilution of FITC-conjugated IgG (Sigma Chemical Company), as previously described by Swalla el til. (1991). The specimens were washed in PBS for 30 min, mounted in 80% glycerol dissolved in PBS, and viewed with an Olympus fluorescence micro- scope. Sections were photographed with Tri X film, ASA 400. Results A large population of M retoriiformis adults was dis- covered living on an underwater hill near Huntsman Ma- rine Station at a depth of 50-100 feet. The animals were attached directly to rocks and lived close to several other ascidian species, Bolicnia ovifera. Molgitla citrina, Ascidia callosti. and Halocynthiapyriformis. The A/, retortiformis adults collected from the underwater hill range
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
