. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 78 L. PIK6, A. TYLER AND J. VINOGRAD able to conclude that the DNA found therein is a component of the yolk spherules. The differences in the relative amounts of DNA obtained from the mitochondria! and yolk fractions in the two experiments may be explained by the sensitivity of these particles to damage during the extraction procedures. The results of cesium chloride buoyant density centrifugation of DNA from mitochondria and from yolk are illustrated in Figure 4a, 41 >. Both DNAs behave similarly, forming bands at somewh
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 78 L. PIK6, A. TYLER AND J. VINOGRAD able to conclude that the DNA found therein is a component of the yolk spherules. The differences in the relative amounts of DNA obtained from the mitochondria! and yolk fractions in the two experiments may be explained by the sensitivity of these particles to damage during the extraction procedures. The results of cesium chloride buoyant density centrifugation of DNA from mitochondria and from yolk are illustrated in Figure 4a, 41 >. Both DNAs behave similarly, forming bands at somewhat higher density than the added radioactively labeled nuclear DNA. In a preparation made by Dr. E. R. Berger, now of the Veterans Administra- tion Hospital, Sepulveda, approximately 2140 mitochondria and 2280 yolk spherules were counted on a montage of electron micrographs of a thin section (maximum diameter) of an egg of L. pictns. From these figures, and values of microns for the diameter of a yolk spherule and equivalent spherical diameter of micron OPTICAL DENSITY AT 260 m^. (a). COUNT/MIN IN I5X 150- °-D-260 COUNT/MIN 100^ 50- OPTICAL DENSITY AT 260 m/x COUNT/MIN IN I5X 150- , '260 COUNT/MIN (b). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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