. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Ixodidae 251 Engorging of Larvae. The host rabbit is placed in 68 count white muslin bag 14" by 18" in size. The larvae from 4 oviposition vials are quickly placed in the ears and about the head of each animal and the bag securely tied. The bagged animals are then placed in cages sup- ported on frames and racks as illustrated in Figure 53. The cage frames 52. Fig. 53.—Diagram of cage rack and cages used in rearing of large numbers of larvae and nymph


. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Ixodidae 251 Engorging of Larvae. The host rabbit is placed in 68 count white muslin bag 14" by 18" in size. The larvae from 4 oviposition vials are quickly placed in the ears and about the head of each animal and the bag securely tied. The bagged animals are then placed in cages sup- ported on frames and racks as illustrated in Figure 53. The cage frames 52. Fig. 53.—Diagram of cage rack and cages used in rearing of large numbers of larvae and nymphs. Capacity of rack is 12 cages. are made of %" wrought iron rods welded in place, painted with alu- minum, and are 15" x 17" by 18" high. Twenty-four hours later the animals are released and the bags and the unattached ticks clinging to them burned. A 10 ounce canvas bottomed bag with an 80 count muslin top is then placed over the supporting frame containing the cage and tied. The canvas bottom has a few small perforations in it to permit drainage into sawdust filled trays below. The frame within which the cage is supported serves to keep the bag from actual contact with the cage thus preventing holes from being gnawed in the cage bag by the tick host. When the feeding is done at room temperature the major portion of the larvae complete engorgement and drop in 5 days. Tem- peratures above or below shorten or lengthen the feeding Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Galtsoff, Paul Simon, 1887-. update forthcoming


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Keywords: ., bookcentury1900, bookcollectionameri, bookcollectionbiodiversity