. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 224 The Cultivation of Anaerobic Organisms ' "Two lengths of nichrome wire 6 cm. long, are separately fused into a glass tube so that thev are insulated (A), and the glass tube (B), closed at each end is passed through a one-hole rubber stopper (C). To the lower ends of the nichrome wire is attached a coil of fine (No. 31) nichrome wire (D) thus completing the circuit. In the coils of the fine wire is placed a small mass of platinized asbestos (E).
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 224 The Cultivation of Anaerobic Organisms ' "Two lengths of nichrome wire 6 cm. long, are separately fused into a glass tube so that thev are insulated (A), and the glass tube (B), closed at each end is passed through a one-hole rubber stopper (C). To the lower ends of the nichrome wire is attached a coil of fine (No. 31) nichrome wire (D) thus completing the circuit. In the coils of the fine wire is placed a small mass of platinized asbestos (E). The apparatus is placed in a package and autoclaved. A large test-tube, 20 by cm. is used, to which 10 cc. of media are added, sterilized and slanted. The water of condensation is removed and the tube inoculated. The tube is then inverted, the cotton plug removed, and the tube filled with hydrogen by means of a sterile capillary pipette. The platinized asbestos mass is heated for a moment in a free flame and the rubber stopper is then firmly inserted into the inverted tube and the end of the tube dipped in melted _Gl435bulb conTdinin^ platinized afbeslos i' Fig. 65.—Detail of the platinized asbestos bulb for the anaerobic jar (Smillie). The tube may now be placed in an upright position and sufl&cient electric current applied to the free ends of the wire to heat the fine nichrome wire wrapped about the platinized asbestos to a red heat. The catalyzer is then heated and the free oxygen and hydrogen unite to forin water. The tube is set aside for one-half hour to an hour, then the platin- ized asbestos is reheated in order to ignite any residual oxygen. The tube may now be incubated. The method is very useful for growing all anaerobes for the oxygen is always removed, whereas the Laidlaw method frequently fails. It is particularly useful for the cultivation of the stricter ; The method can be adapted to Blake bottles and flasks. B
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
