. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MID-ATLANTIC RIDGE MUSSELS 267 Table II En-yme buffer combinations used in ullozyinc electrophoresis En/yme Loci Butter x\stem Aspartale aminotransterase Aat B Isocitarte dehydrogenase hth B Leucme aminopeptidase Lap C and D Malate dehydrogenase Mdh-\. Milh-2 A Malic cn/yme Me B Mannose-6-phosphate isomerase Mpi D D-Octopine dehydrogenase Opdh C Peptidase LG. LGG Pep-g\. Pep-\gg A and D Phoxphoglucose isomerase Pgi A and D Phosphoglucomutase Pgm B and D 6-Phosphogluconate dehydrogenase Pgd B and D Superoxide dismutase Sod C
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MID-ATLANTIC RIDGE MUSSELS 267 Table II En-yme buffer combinations used in ullozyinc electrophoresis En/yme Loci Butter x\stem Aspartale aminotransterase Aat B Isocitarte dehydrogenase hth B Leucme aminopeptidase Lap C and D Malate dehydrogenase Mdh-\. Milh-2 A Malic cn/yme Me B Mannose-6-phosphate isomerase Mpi D D-Octopine dehydrogenase Opdh C Peptidase LG. LGG Pep-g\. Pep-\gg A and D Phoxphoglucose isomerase Pgi A and D Phosphoglucomutase Pgm B and D 6-Phosphogluconate dehydrogenase Pgd B and D Superoxide dismutase Sod C Buffer A (AP ), B (TC ). C (TVB ) and D (TC ). lation and amino acid alignments were performed in ESEE (Cabot and Beckenbach, 1989). Genetic distances were cal- culated in MEGA (Kumar et al., 1994). Due to a large bias in nucleotide base composition, pairwise nucleotide se- quence divergence was estimated with the Tajima-Nei dis- tance algorithm (Tajima and Nei. 1984). GenBank acces- sion numbers are AF128534 and API28533 for haplotypes Al (B. n. sp.) and Bl (B. puteoserpentis), respectively. Restriction fragment length polymorphism analysis The sequences were used to identify a restriction enzyme, Cac& I. which produced diagnostic restriction band patterns for the two mitochondrial haplotypes Al and Bl. ND4 product was obtained by PCR as described above: 10 /nl was digested with 1 /nl of CflfS I and 2 /zl of the appropriate buffer (New England Biolabs, Beverly, MA) and 7 sterile distilled water for a total volume of 20 jul for 1 h at 37°C. Digestions were separated by electrophoresis on a 2% aga- rose gel containing ethidium bromide. The gel was viewed and photographed under UV light. Allo-ymes About g of tissue from the mantle was removed and homogenized in two volumes of grinding buffer ( M Tris/20% glycerol. pH ) and spun at 15,000 X g for 10 min to remove tissue debris. The supernatant fluid was inserted into a preformed well in a sta
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
