. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GIANT AXONS AND ESCAPE SWIMMING 249. Figure 1. Eitplokamix Jitnlapae swimming in an aquarium, oral end up. Scale bar: 5 mm. in 1% osmium tetroxide in the same buffer at 4°C for 1 h. Specimens invariably disintegrated during fixation de- spite every precaution. Fortunately, intact fragments of comb rows, along with some attached underlying tissue, could be retrieved from the debris and processed for elec- tron microscopy. The tissue was dehydrated through graded ethanols and propylene oxide and embedded in Epon 812. Thick sec
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GIANT AXONS AND ESCAPE SWIMMING 249. Figure 1. Eitplokamix Jitnlapae swimming in an aquarium, oral end up. Scale bar: 5 mm. in 1% osmium tetroxide in the same buffer at 4°C for 1 h. Specimens invariably disintegrated during fixation de- spite every precaution. Fortunately, intact fragments of comb rows, along with some attached underlying tissue, could be retrieved from the debris and processed for elec- tron microscopy. The tissue was dehydrated through graded ethanols and propylene oxide and embedded in Epon 812. Thick sections were stained with Richardson's stain. Thin sections were treated with uranyl acetate and lead citrate. Because the tissues were extremely fragile, we could not prepare whole mounts of living material for examination by Nomarski or phase contrast microscopy. Figure 2, showing giant axons in an intact, living animal, was taken through a dissecting microscope illuminated with a double substage mirror, with the mirror angles ar- ranged to give shadows along the edges of the axons. Behavior of free-swimming specimens was observed in a 15-1 tank, illuminated from the sides, and with a dark background. A Sony CCD video camera (HVM-200), fit- ted with a Nikon 105 mm macro lens, was used in con- junction with a VCR with frame-by-frame playback for analysis of responses. Recordings of electrical activity were made from specimens pinned down in a Sylgard-lined Pyrex pie dish placed on top of a doughnut-shaped Cam- bion cooling stage, which allowed light to enter from be- low. Temperature was maintained at 12°C. Fine polyeth- ylene suction electrodes were attached directly to the body surface using minimal suction to keep them attached. Signals were amplified with capacity-coupled amplifiers and displayed on a digital oscilloscope; conventional ex- tracellular recording procedures were used. Stimuli were delivered through paired metal wires insulated to near the tip. Movement of co
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
