. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CA-BLOCKERS AND SPERM MOTILITY 497 150-. -» x p Ouabain FIGURE 3. Time- and dose-dependent effects of ouabain on Arbacia sperm in FSW and MBL- ASW. X's, after 10 min exposure in FSW. Open symbols, in MBL-ASW: circles, 10 min; triangles, 20 min; squares, 30 min after dilution. Ordinate, relative motility in percent of control rate; abscissa, negative log of molar concentration of ouabain in the medium. experiment (Fig. 4), the peak in 10 6 M propranolol is succeeded by a sharp motility decline with increasing drug concentrati
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CA-BLOCKERS AND SPERM MOTILITY 497 150-. -» x p Ouabain FIGURE 3. Time- and dose-dependent effects of ouabain on Arbacia sperm in FSW and MBL- ASW. X's, after 10 min exposure in FSW. Open symbols, in MBL-ASW: circles, 10 min; triangles, 20 min; squares, 30 min after dilution. Ordinate, relative motility in percent of control rate; abscissa, negative log of molar concentration of ouabain in the medium. experiment (Fig. 4), the peak in 10 6 M propranolol is succeeded by a sharp motility decline with increasing drug concentration. Figure 5a shows that prolonging the incubation in propranolol has little added effect at the lower concentrations, but at micromolar amounts stimulation becomes evident and inhibition is somewhat ame- liorated at concentrations of 10~5 M and higher. Incubation in quinidine alone in FSW (shown in Fig. 5b) over a range of con- centrations from 10~'° M to 10~4 M evokes a somewhat uneven but insignificant oscillation around the control rate of movement. The delayed effect on motility does not deviate strikingly from that of the delayed controls when the "dilution" effect is taken into account, viz., a 60% to 80% decrease in progressive movement which is sustained over the entire concentration range after an additional ten minutes of incubation. When the two drugs are tested for interactive effects, the samples incubated in 10"3 M quinidine responded more vigorously than those in 10"5 M. In these experiments, the Arbacia sperm cells in seawater suspension were prein- cubated for five minutes, and, after their motility was rated, to each cuvette was added ml FSW in the single treatment labeled "Q" or "P" or ml of quinidine for the co-incubation, double-treatment series, labeled "P + Q" in the bar graph diagrams (Figs. 6a & 6b). After the additions, the cuvettes were again inverted 2 times to assure uniform redistribu
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