. The microscope; an introduction to microscopic methods and to histology. Microscopes. iSg PREPARATION OF REAGENTS \CH. VII. Fig. 167. Slide box for 25 speci- mens. These are cheap and convenient and may be stood on end like books, thus placing the slides in a horizontal position. Smaller boxes, i. 3, 6 and 12 slides are also made, and mail- ing boxes for a single slide (Bausch & Lomb Co.). SOME REAGENTS FOR FIXING, MOUNTING, ETC. I 312. Albumen Fixative (Mayer's).—This consists of equal parts of well- beaten white of egg and glycerin. To each 50 cc. of this 1 gram of salicylate of
. The microscope; an introduction to microscopic methods and to histology. Microscopes. iSg PREPARATION OF REAGENTS \CH. VII. Fig. 167. Slide box for 25 speci- mens. These are cheap and convenient and may be stood on end like books, thus placing the slides in a horizontal position. Smaller boxes, i. 3, 6 and 12 slides are also made, and mail- ing boxes for a single slide (Bausch & Lomb Co.). SOME REAGENTS FOR FIXING, MOUNTING, ETC. I 312. Albumen Fixative (Mayer's).—This consists of equal parts of well- beaten white of egg and glycerin. To each 50 cc. of this 1 gram of salicylate of soda is added to prevent putrefactive changes. This must be carefully filtered. For method of use see 'i 290. I 313. Alcohol (Ethylic).—Ethyl alcohol is mostly used for histological pur- poses. (A) absolute alcohol (/. e., alcohol of j%%%) is recommended for many purposes, but if plenty of 95% alcohol is used it answers every purpose in histology, in a dry climate or in a warm, dry room. When it is damp dehydration is greatly facilitated by the use of absolute alcohol. (B) 82% alcohol made by mixing 5 parts of 95% alcohol with 1 part of water. (C) 67% alcohol made by mixing 2 parts of 95% alcohol with 1 part of water. Grades of Alcohol. It has been found by careful tests that quite accurate percentages of alcohol may be obtained by mixing water and alcohol as follows : Pour alcohol into a graduate until the volume of alcohol corresponds to the de- sired percentage. Add water until the volume in cubic centimeters corresponds to the original percentage of the alcohol used. For example, to get 67°(, from 95% alcohol, pour 67 cc. of 95% into a graduate, and add sufficient water to bring the volume up to 95 cc. Far 50% alcohol from 75%, put 50 cc. of 75% alcohol in a graduate, add sufficient water to make the volume 75 cc. From the change in volume it does not answer to mix given volumes of water and alcohol in these cases. In the first case, if one mixed 75 cc. of 95% alcohol
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