. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1C 1D. Figure 1A-E. Nomarski micrographs of gallocyanin/chromalum stained whole mounts of eggs in prometaphase (A), metaphase (B). ana- phase (C), telophase (D) and during the extrusion of the first polar body (E). The eggs were fixed at 9, 39, 49, 59, and 60 min after oviposition for A, B, C, D. and E. respectively. The timing concerns development of decapsulated eggs in CFTW + 2 rrtW CaCl2. Treatment with calcium channel blockers Diltiazem. In eggs incubated from about anaphase of the first meiotic cycle in copper-free tap
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1C 1D. Figure 1A-E. Nomarski micrographs of gallocyanin/chromalum stained whole mounts of eggs in prometaphase (A), metaphase (B). ana- phase (C), telophase (D) and during the extrusion of the first polar body (E). The eggs were fixed at 9, 39, 49, 59, and 60 min after oviposition for A, B, C, D. and E. respectively. The timing concerns development of decapsulated eggs in CFTW + 2 rrtW CaCl2. Treatment with calcium channel blockers Diltiazem. In eggs incubated from about anaphase of the first meiotic cycle in copper-free tapwater containing 2 mA/ Cad; and 2 mAI diltiazem, the extrusion of the first polar body was generally complete and at the time of second polar body formation there was a bulging out of cytoplasm resembling the second polar body. How- ever, this plasm was immediately resorbed and the sec- ond polar body never formed. When CaCl2 was not added to CFTW, first polar body was not completely ex- truded and was subsequently resorbed. A representative measurement of such a treatment is shown in Figure 4A. The current density decreased abruptly from 250 to 50 nA/cm2 within 10% of the cell cycle after perfusion with diltiazem, and within 25% of the cell cycle it reached zero. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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