. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 SACKTOR, THOMAS, MOSER AND BLOCK present and is linear with time during the 15-minute incubation period. A repre- sentative set of data is depicted in Figure 1. Furthermore, no inorganic phosphate is liberated either in the absence of homogenate or in the presence of tissue but in the absence of ATP. Determination of protein. Protein was determined by the method of Lowry ct al. (1951). Due to the presence in the fat body of urates, which interfere with the color reaction, aliquots of this tissue homogenate were first ext
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 168 SACKTOR, THOMAS, MOSER AND BLOCK present and is linear with time during the 15-minute incubation period. A repre- sentative set of data is depicted in Figure 1. Furthermore, no inorganic phosphate is liberated either in the absence of homogenate or in the presence of tissue but in the absence of ATP. Determination of protein. Protein was determined by the method of Lowry ct al. (1951). Due to the presence in the fat body of urates, which interfere with the color reaction, aliquots of this tissue homogenate were first extracted with 200- 150- o o o: o. 100- 0 10 15 MINUTES FIGURE 1. The effect of varying concentrations of muscle homogenate on the course of dephosphorylation of ATP. The following symbols: A, O and • represent the liberation of inorganic phosphate in the presence of ml., ml. and ml., respectively. acetone and subsequently the protein was precipitated twice with trichloroacetic acid. Crystalline bovine serum albumin was used as the standard protein. RESULTS The relative capabilities of various cockroach tissues, male and female, in de- phosphorylating ATP, and the influence of bivalent cations on this reaction are shown in Tables I and II. Dephosphorylation by tissues. The data in Tables I and II reveal that insect tissues differ in their ability to dephosphorylate ATP. In general, based on their activity, they can be grouped into three categories. These are (1) those with greatest activity: muscle, fat body and Malpighian tubes; (2) those with moderate. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfie
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
