. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 420 ARNOLD LAZAROW ET AL. The effect of tonicity. In order to study the effect of tonicity, the oxygen up- take of islet slices was measured in media of varying salt concentrations. However, since the phosphate buffer concentration that gives maximal stimulation ( M) has a tonicity equivalent to M NaCl, it was necessary to reduce the phosphate ion to a suboptimal value if lower tonicities were to be used. Therefore additional OXYGEN UPTAKE m/j I 02 PER >jg PROTEIN PER HOUR I .02 .04 PHOSPHATE .
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 420 ARNOLD LAZAROW ET AL. The effect of tonicity. In order to study the effect of tonicity, the oxygen up- take of islet slices was measured in media of varying salt concentrations. However, since the phosphate buffer concentration that gives maximal stimulation ( M) has a tonicity equivalent to M NaCl, it was necessary to reduce the phosphate ion to a suboptimal value if lower tonicities were to be used. Therefore additional OXYGEN UPTAKE m/j I 02 PER >jg PROTEIN PER HOUR I .02 .04 PHOSPHATE .06 .08 .10 .12 CONCENTRATION (M/*) .14 FIGURE 2. Effect of phosphate ion concentration on the respiration of toadfish islet slices. The tonicity of the medium was maintained equivalent to that of M NaCl; pH = Each of the points represents the average of 32 to 161 individual determinations. studies were carried out with lower phosphate concentrations. Where necessary the tonicity was adjusted by adding NaCl. Table VI shows that the oxygen uptake increased with decreasing tonicity. When the tonicity of the medium was equivalent to M NaCl, the metabolic activity of islet slices was 60% greater (p = ) tonicity. Thus the appearance of a maximum at a phosphate concentration of M may be more apparent than Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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