. The microscope : an introduction to microscopic methods and to histology. Microscopes. CH. X] SERIAL SECTIONING 3'9 § 474. Numbering the Serial slides.—For temporary num- bering a fine pen with Higgins' waterproof carbon ink serves well. If the slide is clean one can write on it as well as on paper. When the ink is dry it should be coated with thin shellac or with thin xylene balsam. Sometimes thin collodion is used. It is also im- portant to write the number of the slide with a writing diamond. The double marking is desirable because with wet slides the dia- mond number is hard to see, whil
. The microscope : an introduction to microscopic methods and to histology. Microscopes. CH. X] SERIAL SECTIONING 3'9 § 474. Numbering the Serial slides.—For temporary num- bering a fine pen with Higgins' waterproof carbon ink serves well. If the slide is clean one can write on it as well as on paper. When the ink is dry it should be coated with thin shellac or with thin xylene balsam. Sometimes thin collodion is used. It is also im- portant to write the number of the slide with a writing diamond. The double marking is desirable because with wet slides the dia- mond number is hard to see, while the ink marks are clearly visible. One is not so liable to wipe off the sections if the ink mark is present. Fig. 254. Egg pipette. This is made by putting a short piece of soft rubber tubing over the end of a glass pipette with rubber bulb. With this one can handle the eggs both fresh and hardened without any danger of injury, (four. Appl. Micr. 1S9S, p. 120.) Fig. 255. Lens holder. A lens in such a holder is very convenient for sorting and orienting small eggs or em- bryos in imbedding. One can have the eggs in a watch-glass of melted paraffin on a copper warming plate (Fig. 241) and arrange the eggs or embryos under a lens in such a lens holder. Then if cold ivater is poured on the plate around the watch-glass the paraffin 'will cool and hold them in place. (Cut loaned by the Bausch & Lomb Opt. Co.) FIXING AND STAINING FOR SERIES. § 475. Fixing.—The two most used fixers for embryos are Zenker's fluid and Formaldehyde (§406, 429)- For those unskilled in microscopic technic, or for one who is exceedingly busy the best results are obtained by putting the embryos in formaldehyde, (10 parts of formalin, the formalin of the pharmacy, and 90 parts water answers well). If there is plenty of this the embryos are likely to. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance
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