Archive image from page 11 of Design and operation of a. Design and operation of a carbon-14 biosynthesis chamber . designoperationo911smit Year: 1962 PRESSURE EQUALIZATION TUBE (COPPER) STAINLESS STEEL TUBE SPAN ZERO ANALYZER CALIBRATION GAS CYLINDERS Figttbe schematic diagram of the carbon dioxide control equipment for the biosynthesis chamber. reaction vessel was constructed from a 200-ml. round bottom flask with a side-arm outlet and a ground glass joint inlet with stopcock. A weighed quantity of C-14 barium carbonate was placed in the flask, the inlet and outlet systems connected, a
Archive image from page 11 of Design and operation of a. Design and operation of a carbon-14 biosynthesis chamber . designoperationo911smit Year: 1962 PRESSURE EQUALIZATION TUBE (COPPER) STAINLESS STEEL TUBE SPAN ZERO ANALYZER CALIBRATION GAS CYLINDERS Figttbe schematic diagram of the carbon dioxide control equipment for the biosynthesis chamber. reaction vessel was constructed from a 200-ml. round bottom flask with a side-arm outlet and a ground glass joint inlet with stopcock. A weighed quantity of C-14 barium carbonate was placed in the flask, the inlet and outlet systems connected, and a mixture of sulfuric acid and fuming sulfuric acid admitted dropwise through the stopper in the top. A slight vacuum was applied to sweep out the evolved C02, which was captured in a train of bubblers containing sodium hydroxide solution. The radioactive carbonate solution thus prepared was then mixed with a large volume of sodium bicarbonate solution to produce the desired specific activity. This radio- active solution then became the 'stock' from which a given crop of plants derived its carbon. The carbon dioxide concentration in the bio- synthesis chamber was continually monitored. Air from the chamber was pumped through a flow system to an infrared carbon dioxide analyzer and back into the chamber. The C02 content of the air was recorded on a strip chart recorder. (See fig. 9.) A special switch installed on the recorder was set to actuate the controller when a preset C02 concentration was reached. This opened a solenoid for a given time and emptied the contents of a metering vessel into a reaction vessel in the chamber. The reaction vessel con- tained sulfuric acid that reacted to evolve a quan- tity of carbon dioxide. During normal operation 10 C02 content was automatically maintained be- tween and percent. Figure 9 gives a schematic layout of the carbon dioxide control apparatus. This system has proved to be satis- factory and has overcome the major problem
Size: 2068px × 967px
Photo credit: © Actep Burstov / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: 1960, 1962, allison_franklin_e_franklin_elmer_1892_1972, archive, book, bookauthor, bookdecade, bookpublisher, booksponsor, booksubject, bookyear, carbon, drawing, growth_cabinets_and_rooms, growth_plants_, historical, history, illustration, image, mullins_joseph_f_1894_, page, picture, print, reference, smith_j_h_jay_hamilton_1927_, u_s_department_of_agriculture_national_agricultural_, united_states_department_of_agriculture, vintage, washington_d_c_agricultural_research_service_u_s_d
