. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTHORACIC GLANDS OF LEUCOPHAEA 187 tated by the use of supravital dyes with which the prothoracic glands stain conspicu- ously. Specimens having received injections of neutral red or methylene blue solu- tions prior to dissection offer a suitable material for the study of certain morpho- logical features of the gland. For permanent preparations the prothoracic region was fixed in Zenker-formol, Bouin, or Carothers solution. Paraffin sections 3-7 micra thick were stained with the following techniques : Hematoxylin-eosin, Ma
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTHORACIC GLANDS OF LEUCOPHAEA 187 tated by the use of supravital dyes with which the prothoracic glands stain conspicu- ously. Specimens having received injections of neutral red or methylene blue solu- tions prior to dissection offer a suitable material for the study of certain morpho- logical features of the gland. For permanent preparations the prothoracic region was fixed in Zenker-formol, Bouin, or Carothers solution. Paraffin sections 3-7 micra thick were stained with the following techniques : Hematoxylin-eosin, Mallory- azan, Masson's and Lendrum's trichrome, and Wilder's reticulum stains. Isolated glands were incubated at 56° C. in 2 per cent osmic acid solution for several hours and were studied in toto or after paraffin OBSERVATIONS The prothoracic glands of Lcncophaea consist of two bands of tissue which occupy a ventral position in the anterior thorax in close proximity to the prothoracic ganglion. The two bands cross in the manner indicated in Figure 1 ; at the point of. PROTHORACIC 3r~U PROTHORACIC GANGLION FIGURE 1. Ventral aspect of anterior thorax and head (tilted back) of Leucophaea nymph showing position of prothoracic glands. crossing the organs are connected by a narrow tissue bridge. The anterior ends of the tissue bands taper off in the neck region. At the posterior end each band divides into two short branches one of which establishes nervous connection with the prothoracic ganglion. Figure 2, based on methylene blue preparations, shows a branch of a thin nerve entering the prothoracic gland shortly after its emergence from the lateral surface of the ganglion. No ducts are found in connection with -1 am indebted to Miss Kate Gruen for valuable technical Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly re
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
