. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 244 E. M. LEISE AND M. G. HADFIELD. 5s Figure 2. Representative trace from an isolated head in FSW. Large spikes are truncated and correlate with spontaneous velar ciliary arrest. No stimulus was used to elicit these large ciliary arrest spikes. Bottom trace is manually controlled cue (event marker) on the PCM data recorder. Cue was depressed, yielding an upward deflection, whenever spontaneous velar cilia were observed to cease beating. Audio monitor was turned off to avoid biasing the observer. Cessation of ciliary beating
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 244 E. M. LEISE AND M. G. HADFIELD. 5s Figure 2. Representative trace from an isolated head in FSW. Large spikes are truncated and correlate with spontaneous velar ciliary arrest. No stimulus was used to elicit these large ciliary arrest spikes. Bottom trace is manually controlled cue (event marker) on the PCM data recorder. Cue was depressed, yielding an upward deflection, whenever spontaneous velar cilia were observed to cease beating. Audio monitor was turned off to avoid biasing the observer. Cessation of ciliary beating coincides with the largest spikes. sette tape through an Instrutech VR-100 PCM (pulse code modulation) device. This device has a manually operated event marker, or "cue" switch. When depressed, a positive deflection from ground is recorded on a separate channel on the videotape. Data were played back directly onto a Western Graphtek thermal chart recorder or. alterna- tively, collected on a 486 Insight computer and analyzed with the Enhanced Graphics Acquisition and Analysis (EGAA) software programs, ver. (RC Electronics, Goleta, CA). Action potentials of different magnitudes were identified and counted using the EGAA Waveshape Recog- nition program, which stores start and stop times in digital data tiles. As necessary, files were converted to standard ASCII text format and analyzed further with Microsoft Excel 97 (Microsoft Corp.). Traces with relatively few spikes were analyzed directly from chart recorder records or the EGAA display screens. Two-sample analyses (two- tailed ; tests) were conducted with Statgraphics Plus ver. (Manugistics, Rockville, MD) or GB-STAT (Dy- namic Microsystems. Silver Spring. MD). Results were graphed with DeltaGraph (SPSS, San Francisco, CA). Results Extracellular recordings from the dorsal surfaces of brains in isolated heads of competent veliger larvae dis- played two general sizes of spiking units in
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