Archive image from page 282 of Cytology (1961). Cytology cytology00wils Year: 1961 Cr Manometer Flotation nnediunn Zero line Diver Flotation medium (lithium chloride) Gas space total vol. about 24X 2X Holtfreter solution Tissue Glass tail 'U Fine adjustment -|J Coarse adjustment Figure 11-39. Schematic Diagram of Cartesian Diver Micromanometer. (A) Diver vessel with diver suspended in flotation medium (concentrated lithium chloride solution). The diver vessel is connected to a water manom- eter, the pressure in which can be adjusted by a coarse and fine syringe mechanism (bottom). (B) Det
Archive image from page 282 of Cytology (1961). Cytology cytology00wils Year: 1961 Cr Manometer Flotation nnediunn Zero line Diver Flotation medium (lithium chloride) Gas space total vol. about 24X 2X Holtfreter solution Tissue Glass tail 'U Fine adjustment -|J Coarse adjustment Figure 11-39. Schematic Diagram of Cartesian Diver Micromanometer. (A) Diver vessel with diver suspended in flotation medium (concentrated lithium chloride solution). The diver vessel is connected to a water manom- eter, the pressure in which can be adjusted by a coarse and fine syringe mechanism (bottom). (B) Details of Cartesian diver. The tissue is placed in 2 X (1 X = 1 cu. mm.) of physiological medium at the bottom of the diver bulb which has a glass tail to maintain the diver in a vertical position. The neck is partly occupied by a drop of oil, so placed that there is a small bubble of air between the oil and the flotation medium. A reading is taken by bring- ing the diver to a temporary standstill at the zero line and noting the pressure level in the manometer. (From Boell, E. J., Needham, J., and Peters, V., 1939. 'Morphogenesis and Metabolism: Studies with the Cartesian Diver Ultramicromanometer. 1. Anaerobic Glycolysis of the Regions of the Am- phibian Gastrula,' Proc. Roy. Microscop. Soc. {London), 127, Fig. 1, p. 326.) SURVEY OF CYTOLOGICAL TECHNIQUES / 263
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