. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NUCLEAR PROTEINS FROM THE SPERM OF BIVALVE 35 A. IL. B. mn a b c d e f 9 _ I ?v5SHHBI^HBM^H as a b c d e f 9 _ 16 24 32 TIME ,min. 48 Figure 3. (A) Gel filtration FPLC on a Superose 12 HR 10/30 column. The elution buffer was 6 M Gdn-HCI in 50 mA/ Tris-HCl pH The flow rate was ml/min. The elution profiles of HC1 nuclear extracts from the sperm of Mytilimeria nuttalli ( ) and Agriodesma saxicola ( ) are shown together with the elution profile (••••) of some of the standards used to calibr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NUCLEAR PROTEINS FROM THE SPERM OF BIVALVE 35 A. IL. B. mn a b c d e f 9 _ I ?v5SHHBI^HBM^H as a b c d e f 9 _ 16 24 32 TIME ,min. 48 Figure 3. (A) Gel filtration FPLC on a Superose 12 HR 10/30 column. The elution buffer was 6 M Gdn-HCI in 50 mA/ Tris-HCl pH The flow rate was ml/min. The elution profiles of HC1 nuclear extracts from the sperm of Mytilimeria nuttalli ( ) and Agriodesma saxicola ( ) are shown together with the elution profile (••••) of some of the standards used to calibrate this column: I: PL-I from Spisula solidissima; II: Histone HI from calf thymus; III: PL-HI from Mytilus edulis; IV: protamine salmine; V: vitamin B 12; VI: dansyl-L-alanine. (B) Electrophoretic analysis on urea-acetic acid gels of the fractions a, b. c. d. e, f, g of the elution profiles of At. nuttalli and A. saxicola. mn: starting sample of A/ nuttalli. as: starting sample of A. saxicola. H\ of 4-vinylpyridine was added, and the reaction was al- lowed to proceed for 2 h at room temperature. The sample was then immediately desalted in an HPLC reverse phase C8Vydac column, which was eluted for 5 min with TFA (trifluoroacetic acid), and for 20 min with a 0-70% acetonitrile gradient in TFA. /3-lactoglobulin from Applied Biosystems Inc. was used as a standard for this procedure. Trypsin digestion. Trypsin digestion of proteins in high salt—2 M NaCl, 50 mA/ Na-phosphate buffer (pH )— was carried out as described elsewhere (Ausio el ai, \ 987). Results Chromatographic analysis and purification of the sperm-specific nuclear proteins from A. saxicola and M. nuttalli Figure 1 shows the N HC1 protein extracts from the nuclei of the sperm of A. saxicola and Al. nuttalli. They are shown in comparison to the five groups previously established for the classification of the nuclear sperm-spe- cific proteins of the bivalve mollusks (Ausio, 1986). In each of t
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
