. Clinical diagnostics of the internal diseases of domestic animals. Veterinary medicine; Diagnosis, Veterinary; Veterinary medicine. 192 CLINICAL DIAGNOSTICS. e. Fig. 41. Grape sugar, Glycosuria, by means ordi- narily employed can be detect- ed in urine in disease only, viz. in diabetes m e 11 i t u s. In horses this disease has been observed in a few instances only, in dogs it is common. We suspect the presence of sugar in polyuria when the specific gravity of the urine is high. Chemical determination. If albumin is present this must first be removed by adding acetic acid, boiling, and filte
. Clinical diagnostics of the internal diseases of domestic animals. Veterinary medicine; Diagnosis, Veterinary; Veterinary medicine. 192 CLINICAL DIAGNOSTICS. e. Fig. 41. Grape sugar, Glycosuria, by means ordi- narily employed can be detect- ed in urine in disease only, viz. in diabetes m e 11 i t u s. In horses this disease has been observed in a few instances only, in dogs it is common. We suspect the presence of sugar in polyuria when the specific gravity of the urine is high. Chemical determination. If albumin is present this must first be removed by adding acetic acid, boiling, and filtering. Then add to 30 cc urine 1 cc caustic potash solution; if this produces cloudiness, filter again. Then add about 3 drops of a 10% solution of sul- phate of copper. The appearance of a light blue color is in itself an indication of grape sugar; now heat the fluid, if grape sugar is present an orange yellow precipitate which gradually extends downward is formed at the surface; this is an oxide of copper. Fermentation tube. t^ i_ • i. i. / t< f j. ^ \ This test (Irommer stest) is by no means reliable for horse urine because the latter contains other bodies that have a reducing power: Pyrocatechin, etc. On the other hand, substances that prevent the reduction (or precipitation) of oxide of copper may be present. Pure grape sugar, when added to horse urine, can sometimes not be detected at all by means of Trammer's test. In all cases of doubt we must therefore resort to the fermentation test, as follows: Boil 20 cc of urine that has been freed from albumin, let cool and add a piece of baker's yeast as large as a pea, shake thorough- ly, pour into a fermentation tube and close the latter with metallic mercury. Keep the tube at room temperature for 24-48 hours. If sugar is present fermentation will set in and the CO, thus pro- duced will collect in the top of the tube where the percentage is indicated by a graduated scale. C6 H12 Oa = 2C2 H6 OH2 + CO, Grape sugar = alcohol-4
Size: 1004px × 2489px
Photo credit: © Central Historic Books / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubjectveterin, bookyear1912
