. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 66s tray. The skin is ripped up and turned back. The exposed ab- dominal muscles are now washed with bichlorid solution and a piece of gauze wrung out of the solution temporarily laid on to absorb the excess. With fresh sterile forceps and scissors the abdominal wall is next laid open and fastened back. With fresh sterile instruments the spleen, which should be large and full of tubercles, is drawn forward and, one after another, bits the siz
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 66s tray. The skin is ripped up and turned back. The exposed ab- dominal muscles are now washed with bichlorid solution and a piece of gauze wrung out of the solution temporarily laid on to absorb the excess. With fresh sterile forceps and scissors the abdominal wall is next laid open and fastened back. With fresh sterile instruments the spleen, which should be large and full of tubercles, is drawn forward and, one after another, bits the size of a pea cut or torn off and immediately dropped upon the surface of appropriate culture- media in appropriate tubes. The fragments of tissue from the spleen of the tuberculous guinea-pig are not crushed or comminuted, but are simply laid upon the undisturbed surface of the culture medium and then incubated for several weeks. If no growth is apparent after this period, the bit of tissue is stirred about a little and the tube returned to the incubator, where growth almost immediately begins from bacilli scattered over the surface as the bit of tissue was moved. As the ap- propriate medium, blood-serum was recommended by Koch; glycerin agar- agar, by Roux and Nocard; glycerinized potato, by Nocard; coagulated dogs' blood-serum, by Smith, or coagulated egg, by Dorset, may be mentioned. The most certain results seem to follow the employment of the dogs' serum and egg media. Cultivatio n.—Blood-serum.—Koch first achieved artificial cultivation of the tubercle bacillus upon blood-serum, upon which the bacilli are first appa- rent to the naked eye in about two weeks, in the form of small, dry, whitish flakes, not unlike fragments of chalk. These slowly increase in size at the edges, and gradually form small scale-like masses, which under the microscope are found to consist of tangled masses of baciUi, many of which are in a condition of involution. The medium is so ill adapted t
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
