. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. v-: FIGURE 6. Digestive gland tubules from a starved animal, stained for £J-glucuronidase activity by the method of Fishman and Baker, following fixation in cold chloral-hydrate- formalin. Enzymic activity is confined to the yellow granules. Brown granules (arrows) did not stain for enzymic activity but are emphasized by the yellow filter employed. Calcium cells are negative. 200 X. FIGURE 7. Digestive gland tubule from a recently fed animal, stained as in Figure 6. There is increased cytoplasmic activity, especially at the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. v-: FIGURE 6. Digestive gland tubules from a starved animal, stained for £J-glucuronidase activity by the method of Fishman and Baker, following fixation in cold chloral-hydrate- formalin. Enzymic activity is confined to the yellow granules. Brown granules (arrows) did not stain for enzymic activity but are emphasized by the yellow filter employed. Calcium cells are negative. 200 X. FIGURE 7. Digestive gland tubule from a recently fed animal, stained as in Figure 6. There is increased cytoplasmic activity, especially at the periphery of SR cells. The yellow granules are also stained. Calcium cells are negative. Yellow filter. 200 X. outside the cell due to compression of the sections during processing (Figs. 2 and 3). Application of heat to the sections (90° C. for at least 10 minutes), or omis- sion of glycerophosphate substrate from the incubation medium, demonstrated that this localization of final reaction product was a false positive reaction not due to enzymic activity. With use of an azo dye method for acid phosphatase activity, the spherules showed no enzymic activity (Figs. 4 and 5). With staining by the azo dye method, however, it was clear that the cytoplasm of calcareous cells from recently fed animals showed increased acid phosphatase activity over cells from starved animals (compare Figs. 4 and 5). The SR cells from starved animals showed little or no enzymic activity by either the azo dye or lead-salt methods. In fed animals, some enzymic activity could be detected near the luminal border in the region where the small, colorless. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
