. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. K. L. COOPER, \V. P1XKEKTOX AXi) \V. H. H1LDEAIANN on one side. After a short quick stab into the tail with a sterile lancet, blood wa> collected easily and quickly with capillary pipettes and placed into culture tubes (60x50 mm.). \Vhen the clot had completely retracted after approxi- mately one hour at room temperature, the tubes were ccntrifuged at 1500 rpm r 10 minutes. The serum was then placed in sterile culture tubes, stoppered and stored at -20° C. RESULTS Three of the eight larvae immunized over a two-month p
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. K. L. COOPER, \V. P1XKEKTOX AXi) \V. H. H1LDEAIANN on one side. After a short quick stab into the tail with a sterile lancet, blood wa> collected easily and quickly with capillary pipettes and placed into culture tubes (60x50 mm.). \Vhen the clot had completely retracted after approxi- mately one hour at room temperature, the tubes were ccntrifuged at 1500 rpm r 10 minutes. The serum was then placed in sterile culture tubes, stoppered and stored at -20° C. RESULTS Three of the eight larvae immunized over a two-month period with allogeneic serum yielded serums that gave isoprecipitin bands (Fig. la). Thus, the existence of serum isoantigens or serum groups in this species is evident. The apparent lack of isoprecipitin production in some recipients is attributable either to insuffi- cient immunization or to sharing of major antigens with donor serums. Inasmuch as serums obtained from these larvae prior to the last two injections gave negative results, it appears that prolonged immunization is necessary for substantial iso- precipitin production. o o j o j o o G Q® O ;-0. FIGURE 1 a, h. Characteristic precipitation patterns in double diffusion plates obtained from tadpoles immunized with adult serum (isoimmunization, Fig. 1 a), and adult goldfish serum (Fig. 1 b). Note differences in the precipitin bands between the two groups. Longer periods of immunization were necessary to produce isoprecipitins than precipitins to xenogeneic serum antigens. Six injections of antigen were required when using the allogeneic antigen. The first and fifth consisted of whole adult bullfrog serum emulsified in incomplete Freund's adjuvant. In the case of the xenogeneic group, only two injections were necessary. The first consisted of antigen emulsified in Freund's adjuvant. The data summarized in Table 1 support the thesis that bullfrog larvae are capable of synthesizing serum antibodies to xenogeneic antigens.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
