. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ABSORPTION SPECTRA OF HEMOCYANIN 165 It is consequently desirable to examine the effect of the nature of the solvent upon the absorption of light by hemocyanin solutions. Hydrogen Ion Concentration.—The first point to be considered is the influence of hydrogen ion concentration upon absorption and scat- tering. When specimens of Limulus blood, to which various amounts OB O7. 45O 5OO 55O GOO HAVE LENGTH 650 7OO FIG. 11. Absorption spectra of blood of Limulus polyphcmns at different hydrogen ion concentrations. Upper curve
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ABSORPTION SPECTRA OF HEMOCYANIN 165 It is consequently desirable to examine the effect of the nature of the solvent upon the absorption of light by hemocyanin solutions. Hydrogen Ion Concentration.—The first point to be considered is the influence of hydrogen ion concentration upon absorption and scat- tering. When specimens of Limulus blood, to which various amounts OB O7. 45O 5OO 55O GOO HAVE LENGTH 650 7OO FIG. 11. Absorption spectra of blood of Limulus polyphcmns at different hydrogen ion concentrations. Upper curves, oxygenated blood at pH (hollow circles) and pH (dots) ; lower curves, the same after reduction; intermediate curve, the spectrum of chromatic group, which is identical in both cases. Copper content mgm. per cc.; length of absorption vessel cm. Dilution which gave pH : 20 parts blood, 35 parts sea water. 5 parts NaOH; dilution which gave pH : 20 parts blood, 35 parts sea water, 5 parts NaOH. of acid or alkali have been added, are examined, it is obvious to the eye that the color of the solution more alkaline than about pH 9 is different from the others. This difference is evident not onlv in the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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