. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 76 LANG, COSTELLO AND GOVIND MATERIALS AND METHODS Lobsters (Hoinants ainericanus, Milne-Edwards) were trapped in the local waters around Woods Hole, Massachusetts and kept in ambient running sea water. One large animal (3600 g) was purchased from a local supplier. All animals were held without claw restraints and were fed several times weekly. Animals were weighed several minutes after being taken out of the sea water. No attempt was made to dry them completely. Lengths were measured from the tip of the rostrum to the end o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 76 LANG, COSTELLO AND GOVIND MATERIALS AND METHODS Lobsters (Hoinants ainericanus, Milne-Edwards) were trapped in the local waters around Woods Hole, Massachusetts and kept in ambient running sea water. One large animal (3600 g) was purchased from a local supplier. All animals were held without claw restraints and were fed several times weekly. Animals were weighed several minutes after being taken out of the sea water. No attempt was made to dry them completely. Lengths were measured from the tip of the rostrum to the end of the telson. The closer muscles were prepared in a manner similar to that used by Jahromi and Atwood (1971a). Closer muscles were immobilized by clamping the dactyl in the open position. Perfusion was then accomplished by inserting hypodermic needles through holes in the exoskeleton to ensure delivery of Bouin's fixative to all parts of the muscle. After perfusing for 2-3 hr, the closer muscle was exposed by chipping away the exoskeleton and removing the opener muscle. The claw was subsequently immersed in fresh fixative for several hours. After fixation, the closer muscle was removed and stored in 90% ethanol. To provide a basis on which to sample the closer muscle, the inner aspect of each muscle was divided into nine sections and at least 10 fibers sampled from each section. Previous work had shown the outer aspect to have a similar dis- tribution of fiber types (unpublished observations). Proximal, central and distal areas were identified and each was further subdivided into dorsal, medial and ventral sections, giving a total of nine areas to sample (Fig. 1). Fibers from each section were put into individual vials filled with 90% ethanol. In order to examine the possibility that fiber types were distributed homogeneously within areas, a different sampling procedure was used for the closer muscles of the largest animal (3600 g). Fibers were removed from the outer edges of a
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